Development of nuclear microsatellite markers in Stizophyllum (Bignoniaceae) using next-generation sequencing

Autor(es)

Beyer, Maila ^[1] ; Nazareno, Alison Goncalvez ^[1] ; Lohmann, Lucia G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION; v. 17, n. 4, p. 382-385, AUG 2019.
• Citações Web of Science: 1

Resumo

Here we developed the first set of nuclear microsatellite markers (nSSRs) for Stizophyllum riparium (Bignoniaceae), a widespread species of Neotropical liana. Thirty-two sets of primers were isolated from a genomic dataset obtained with an Illumina HiSeq Platform, and characterized for 57 individuals of S. riparium from three populations. Nine nSSRs were polymorphic and the number of alleles ranged from eight to 29. The unbiased expected heterozygosity per locus ranged from 0.724 to 0.952 and the polymorphic information content values ranged from 0.717 to 0.944. All nine primer pairs also amplified for two closely related species (S. inaequilaterum and S. perforatum). The new set of nuclear markers will be useful for population genetics studies of Stizophyllum as a whole. (AU)

• Processo FAPESP: 13/12633-8 - Filogeografia comparada de plantas na Amazônia Central
• Beneficiário: Alison Gonçalves Nazareno
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/50260-6 - Estruturação e evolução da biota amazônica e seu ambiente: uma abordagem integrativa
• Beneficiário: Lúcia Garcez Lohmann
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático