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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of nuclear microsatellite markers in Stizophyllum (Bignoniaceae) using next-generation sequencing

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Author(s):
Beyer, Maila [1] ; Nazareno, Alison Goncalvez [1] ; Lohmann, Lucia G. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION; v. 17, n. 4, p. 382-385, AUG 2019.
Web of Science Citations: 1
Abstract

Here we developed the first set of nuclear microsatellite markers (nSSRs) for Stizophyllum riparium (Bignoniaceae), a widespread species of Neotropical liana. Thirty-two sets of primers were isolated from a genomic dataset obtained with an Illumina HiSeq Platform, and characterized for 57 individuals of S. riparium from three populations. Nine nSSRs were polymorphic and the number of alleles ranged from eight to 29. The unbiased expected heterozygosity per locus ranged from 0.724 to 0.952 and the polymorphic information content values ranged from 0.717 to 0.944. All nine primer pairs also amplified for two closely related species (S. inaequilaterum and S. perforatum). The new set of nuclear markers will be useful for population genetics studies of Stizophyllum as a whole. (AU)

FAPESP's process: 13/12633-8 - Comparative phylogeography of plants in the Central Amazonia
Grantee:Alison Gonçalves Nazareno
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach
Grantee:Lúcia Garcez Lohmann
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants