Development of nuclear microsatellite markers in Stizophyllum (Bignoniaceae) using next-generation sequencing

Author(s)

Beyer, Maila ^[1] ; Nazareno, Alison Goncalvez ^[1] ; Lohmann, Lucia G. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION; v. 17, n. 4, p. 382-385, AUG 2019.
• Web of Science Citations: 1

Abstract

Here we developed the first set of nuclear microsatellite markers (nSSRs) for Stizophyllum riparium (Bignoniaceae), a widespread species of Neotropical liana. Thirty-two sets of primers were isolated from a genomic dataset obtained with an Illumina HiSeq Platform, and characterized for 57 individuals of S. riparium from three populations. Nine nSSRs were polymorphic and the number of alleles ranged from eight to 29. The unbiased expected heterozygosity per locus ranged from 0.724 to 0.952 and the polymorphic information content values ranged from 0.717 to 0.944. All nine primer pairs also amplified for two closely related species (S. inaequilaterum and S. perforatum). The new set of nuclear markers will be useful for population genetics studies of Stizophyllum as a whole. (AU)

• FAPESP's process: 13/12633-8 - Comparative phylogeography of plants in the Central Amazonia
• Grantee: Alison Gonçalves Nazareno
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach
• Grantee: Lúcia Garcez Lohmann
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants