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Development of molecular markers based on sugarcane ESTs for the selection of economically important characteristics

Grant number: 02/01167-1
Support type:Research Grants - Research Partnership for Technological Innovation - PITE
Duration: July 01, 2002 - September 30, 2008
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Anete Pereira de Souza
Grantee:Anete Pereira de Souza
Home Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Company: Cooperativa de Produtores de Cana-de-Açúcar, Açúcar e Álcool do Estado de São Paulo (COPERSUCAR)
City: Campinas

Abstract

The EST sequencing project (Sugarcane EST Project – Sucest) of the FAPESP-Genome program has already identified around 40 thousand clusters which represent the sugarcane genes. The ESTs have the potential to be used in the development of genetic markers. In this way, microsatellite markers can be obtained derived from EST databases, and EST probes can be used in RFLP trials for the mapping of QTLs. In view of the advances that should be achieved in the genetic improvement of sugarcane with the exploitation of the information contained in the EST databases, the proposal is, beginning with these sequences, to develop molecular markers of the RFLP/ microsatellite type. It is also intended to develop specific markers for agronomic characteristics of interest, via conversion of RFLP markers (hybridized with EST probes) into specific PCR markers (SCARs and STSs). The development of these markers will be integrated into a mapping program of qualitative and quantitative characteristics which is in the process of being developed using an F1 population, obtained from the crossing of two commercial varieties of sugarcane. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PINTO, L. R.; GARCIA, A. A. F.; PASTINA, M. M.; TEIXEIRA, L. H. M.; BRESSIANI, J. A.; ULIAN, E. C.; BIDOIA, M. A. P.; SOUZA, A. P. Analysis of genomic and functional RFLP derived markers associated with sucrose content, fiber and yield QTLs in a sugarcane (Saccharum spp.) commercial cross. EUPHYTICA, v. 172, n. 3, p. 313-327, APR 2010. Web of Science Citations: 23.
OLIVEIRA, K. M.; PINTO, L. R.; MARCONI, T. G.; MOLLINARI, M.; ULIAN, E. C.; CHABREGAS, S. M.; FALCO, M. C.; BURNQUIST, W.; GARCIA, A. A. F.; SOUZA, A. P. Characterization of new polymorphic functional markers for sugarcane. GENOME, v. 52, n. 2, p. 191-209, FEB 2009. Web of Science Citations: 54.
OLIVEIRA‚ K.M.; PINTO‚ L.R.; MARCONI‚ T.G.; MARGARIDO‚ G.R.A.; PASTINA‚ M.M.; TEIXEIRA‚ L.H.M.; FIGUEIRA‚ A.V.; ULIAN‚ E.C.; GARCIA‚ A.A.F.; SOUZA‚ A.P. Functional integrated genetic linkage map based on EST-markers for a sugarcane (Saccharum spp.) commercial cross. MOLECULAR BREEDING, v. 20, n. 3, p. 189-208, 2007.
R. VICENTINI; M. MENOSSI. Pipeline for macro- and microarray analyses. Brazilian Journal of Medical and Biological Research, v. 40, n. 5, p. 615-619, Maio 2007.
GARCIA, A. A.; KIDO, E. A.; MEZA, A. N.; SOUZA, H. M.; PINTO, LUCIANA ROSSINI; PASTINA, M. M.; LEITE, C. S.; SILVA, J. A.; ULIAN, E. C.; ET AL. Development of an integrated genetic map of a sugarcane (Saccharum spp. ) commercial cross, based on a maximum-likelihood approach for estimation of linkage and linkage phases. THEORETICAL AND APPLIED GENETICS, v. 112, n. 2, p. 298-314, Jan. 2006.
PINTO‚ LR; OLIVEIRA‚ KM; MARCONI‚ T.; GARCIA‚ AAF; ULIAN‚ EC; DE SOUZA‚ AP. Characterization of novel sugarcane expressed sequence tag microsatellites and their comparison with genomic SSRs. PLANT BREEDING, v. 125, n. 4, p. 378-384, 2006.
DRUMMOND, R. D.; PINHEIRO, A.; ROCHA, C. S.; MENOSSI, M. ISER: selection of differentially expressed genes from DNA array data by non-linear data transformations and local fitting. Bioinformatics, v. 21, n. 24, p. 4427-4429, Dec. 2005.

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