Development of molecular markers derived from ESTs in sugarcane for selection of e...
| Grant number: | 02/01167-1 |
| Support type: | Research Grants - Research Partnership for Technological Innovation - PITE |
| Duration: | July 01, 2002 - September 30, 2008 |
| Field of knowledge: | Agronomical Sciences - Agronomy |
| Principal Investigator: | Anete Pereira de Souza |
| Grantee: | Anete Pereira de Souza |
| Home Institution: | Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Company: | Cooperativa de Produtores de Cana-de-Açúcar, Açúcar e Álcool do Estado de São Paulo (COPERSUCAR) |
| City: | Campinas |
Abstract
The EST sequencing project (Sugarcane EST Project – Sucest) of the FAPESP-Genome program has already identified around 40 thousand clusters which represent the sugarcane genes. The ESTs have the potential to be used in the development of genetic markers. In this way, microsatellite markers can be obtained derived from EST databases, and EST probes can be used in RFLP trials for the mapping of QTLs. In view of the advances that should be achieved in the genetic improvement of sugarcane with the exploitation of the information contained in the EST databases, the proposal is, beginning with these sequences, to develop molecular markers of the RFLP/ microsatellite type. It is also intended to develop specific markers for agronomic characteristics of interest, via conversion of RFLP markers (hybridized with EST probes) into specific PCR markers (SCARs and STSs). The development of these markers will be integrated into a mapping program of qualitative and quantitative characteristics which is in the process of being developed using an F1 population, obtained from the crossing of two commercial varieties of sugarcane. (AU)