HIV-1 genetic diversity and divergence and its correlation with disease progression among antiretroviral naive recently infected individuals

Autor(es)

Leda, Ana Rachel ^[1] ; Hunter, James ^[1] ; de Oliveira, Ursula Castro ^[2] ; de Azevedo, Inacio Junqueira ^[2] ; Kallas, Esper G. ^[3] ; Araripe Sucupira, Maria Cecilia ^[1] ; Diaz, Ricardo Sobhie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Sao Paulo - Brazil

^[2] Butantan Inst, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 541, p. 13-24, FEB 2020.
• Citações Web of Science: 0

Resumo

HIV-1 genetic diversity evolution was deeply characterized during the first year of infection among recently-infected patients using deep sequencing technology and correlated with disease progression surrogate markers. RNA and DNA samples from twenty-five individuals (13 female) encoding the protease and reverse transcriptase regions of the poi gene, and the V3 region of the env gene were evaluated at recent infection and during established infection. Infection by a unique HIV-1 strain was inferred in 70.1% of the individuals, with no differences between genders. Infections by multiple strains were associated with higher viral loads and faster CD4(+) T cell declines. Either low or high levels of viral loads accompanied low levels of genetic diversity and lower selective pressure. With massive sequence data from 3 distinct genomic HIV-1 regions from plasma and PBMCs over time, we propose a model for HIV-1 genetic diversity, which correlates to basal viral loads of patients. (AU)

• Processo FAPESP: 11/50238-8 - Estudos das quasispecies de hiv com uso do sequenciamento paralelo macico.
• Beneficiário: Ana Rachel Oliveira Léda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/12156-0 - Estudos das quasispecies do HIV com o uso de sequenciamento paralelo maciço
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Regular