| Full text | |
| Author(s): |
Leda, Ana Rachel
[1]
;
Hunter, James
[1]
;
de Oliveira, Ursula Castro
[2]
;
de Azevedo, Inacio Junqueira
[2]
;
Kallas, Esper G.
[3]
;
Araripe Sucupira, Maria Cecilia
[1]
;
Diaz, Ricardo Sobhie
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Sao Paulo - Brazil
[2] Butantan Inst, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | VIROLOGY; v. 541, p. 13-24, FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
HIV-1 genetic diversity evolution was deeply characterized during the first year of infection among recently-infected patients using deep sequencing technology and correlated with disease progression surrogate markers. RNA and DNA samples from twenty-five individuals (13 female) encoding the protease and reverse transcriptase regions of the poi gene, and the V3 region of the env gene were evaluated at recent infection and during established infection. Infection by a unique HIV-1 strain was inferred in 70.1% of the individuals, with no differences between genders. Infections by multiple strains were associated with higher viral loads and faster CD4(+) T cell declines. Either low or high levels of viral loads accompanied low levels of genetic diversity and lower selective pressure. With massive sequence data from 3 distinct genomic HIV-1 regions from plasma and PBMCs over time, we propose a model for HIV-1 genetic diversity, which correlates to basal viral loads of patients. (AU) | |
| FAPESP's process: | 11/12156-0 - HIV quasispecies studies using ultra-deep sequencing |
| Grantee: | Ricardo Sobhie Diaz |
| Support Opportunities: | Regular Research Grants |