Increased Mmp/Reck Expression Ratio Is Associated with Increased Recognition Memory Performance in a Parkinson's Disease Animal Model

Autor(es)

Spindola, Adauto ^[1] ; Targa, Adriano D. S. ^{[2, 3]} ; Rodrigues, Lais Soares ^{[2, 3]} ; Winnischofer, Sheila Maria Brochado ^{[4, 5]} ; Lima, Marcelo M. S. ^{[2, 3]} ; Sogayar, Mari Cleide ^{[1, 6]} ; Trombetta-Lima, Marina ^[1]

Afiliação do(s) autor(es)

^[1] Universidade Sao Paulo, Faculdade Medicina, Nucleo Terapia Celular Mol, NUCEL, BR-05360-130 Sao Paulo, SP - Brazil

^[2] Universidade Fed Parana, Departamento Fisiol, Laboratorio Neurofisiol, BR-81531-990 Curitiba - Brazil

^[3] Universidade Fed Parana, Departamento Farmacol, BR-81531-990 Curitiba - Brazil

^[4] Universidade Fed Parana, Departamento BioQuim Biol Mol, BR-81531-990 Curitiba - Brazil

^[5] Universidade Fed Parana, Departamento Biol Celular Mol, BR-81531-990 Curitiba - Brazil

^[6] Universidade Sao Paulo, Departamento BioQuim, Instituto Quim, BR-05508-000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Neurobiology; v. 57, n. 2, p. 837-847, FEB 2020.
• Citações Web of Science: 0

Resumo

Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder worldwide. Among its non-motor symptoms, sleep disorders are extremely common, being linked to cognitive and memory disruption. The microenvironment, particularly the extracellular matrix (ECM), is deeply involved in memory consolidation as well as in neuropathological processes, such as inflammation, damage to the blood-brain barrier and neuronal death. To better understand ECM dynamics in PD memory disturbances, we investigated the orchestrated expression of Mmps (Mmp-3, Mmp-7, and Mmp-9) and their modulators (Reck and Timp-3) in a rotenone-induced PD model. Also, we introduced an additional intervention in the memory process through rapid eye movement sleep deprivation (REMSD). We observed a REMSD-induced trend in reversing the memory impairment caused by rotenone administration. Associated to this phenotype, we observed a significant increase in Mmp-7/Reck and Mmp-9/Reck mRNA expression ratio in the substantia nigra and Mmp-9/Reck ratio in the hypothalamus. Moreover, the positive correlation of Mmp/Reck expression ratios between the substantia nigra and the striatum, observed upon rotenone infusion, was reversed by REMSD. Taken together, our results suggest a potential orchestrated association between an increase in Mmp-7 and Mmp-9/Reck expression ratios in the substantia nigra and a possible positive effect on cognitive performance in subjects affected by PD. (AU)

• Processo FAPESP: 16/18277-7 - Análise do papel do gene supressor de tumor Reck em divisões celulares assimétricas
• Beneficiário: Marina Trombetta Lima
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/05311-2 - Medicina regenerativa visando à terapia de doenças crônico-degenerativas (câncer e diabetes)
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/26328-8 - Análise comparativa do papel do gene supressor de tumor RECK na sub-população de células-tronco tumorais de GBM e em precursores neurais normais.
• Beneficiário: Marina Trombetta Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado