| Texto completo | |
| Autor(es): |
Rodrigues, Marili V. N.
[1]
;
Rodrigues-Silva, Caio
[2]
;
Boaventura Jr, Sinesio
;
Oliveira, Adriana S. S.
[3]
;
Rath, Susanne
[2]
;
Cass, Quezia B.
[4]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim, UNICAMP, Div Quim Organ & Farmaceut, Ctr Pluridisciplinar P, BR-13148218 Paulinia, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, Campinas, SP - Brazil
[3] Boaventura Jr, Jr., Sinesio, Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim, UNICAMP, Div Quim Organ & Farmaceut, Ctr Pluridisciplinar P, BR-13148218 Paulinia, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Quim, Cx Postal 676, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Pharmaceutical and Biomedical Analysis; v. 181, MAR 20 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The screening of compounds is the initial step in research for the development of new drugs. For this reason, the availability of fast and reliable tools for the screening of a large number of compounds becomes essential. Among the therapeutic targets, the enzyme xanthine oxidase (XO) is of great interest for its importance as a biological source of superoxide radicals, which contribute to the oxidative stress on organisms and are involved in many pathological processes. In the present study, we validated a new method using an immobilized capillary enzyme reactor in an LC system directly coupled to triple quadrupole mass spectrometry to screen for XO ligands. The use of mass spectrometry provided selectivity and speed to the system, eliminating the analytical separation step. The Michaelis-Menten constant (K-M) value determined for the immobilized enzyme was 14.5 +/- 0.4 mu mol L-1, which is consistent with the value previously reported for the XO-ICER with UV detection in a 2D LC method. The on-line approach was successfully applied to assay the XO inhibitory activities of thirty isolated compounds from different classes of natural products and provided greater productivity (288 analysis/day) than 2D LC method (84 analysis/day) of screened samples. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 16/24087-6 - Busca de inibidores da xantina oxidase em amostras de origem vegetal empregando diferentes versões da biocromatografia |
| Beneficiário: | Marili Villa Nova Rodrigues |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |