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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

On-Flow LC-MS/MS method for screening of xanthine oxidase inhibitors

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Author(s):
Rodrigues, Marili V. N. [1] ; Rodrigues-Silva, Caio [2] ; Boaventura Jr, Sinesio ; Oliveira, Adriana S. S. [3] ; Rath, Susanne [2] ; Cass, Quezia B. [4]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim, UNICAMP, Div Quim Organ & Farmaceut, Ctr Pluridisciplinar P, BR-13148218 Paulinia, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, Campinas, SP - Brazil
[3] Boaventura Jr, Jr., Sinesio, Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim, UNICAMP, Div Quim Organ & Farmaceut, Ctr Pluridisciplinar P, BR-13148218 Paulinia, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Quim, Cx Postal 676, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Pharmaceutical and Biomedical Analysis; v. 181, MAR 20 2020.
Web of Science Citations: 0
Abstract

The screening of compounds is the initial step in research for the development of new drugs. For this reason, the availability of fast and reliable tools for the screening of a large number of compounds becomes essential. Among the therapeutic targets, the enzyme xanthine oxidase (XO) is of great interest for its importance as a biological source of superoxide radicals, which contribute to the oxidative stress on organisms and are involved in many pathological processes. In the present study, we validated a new method using an immobilized capillary enzyme reactor in an LC system directly coupled to triple quadrupole mass spectrometry to screen for XO ligands. The use of mass spectrometry provided selectivity and speed to the system, eliminating the analytical separation step. The Michaelis-Menten constant (K-M) value determined for the immobilized enzyme was 14.5 +/- 0.4 mu mol L-1, which is consistent with the value previously reported for the XO-ICER with UV detection in a 2D LC method. The on-line approach was successfully applied to assay the XO inhibitory activities of thirty isolated compounds from different classes of natural products and provided greater productivity (288 analysis/day) than 2D LC method (84 analysis/day) of screened samples. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 16/24087-6 - Search xanthine oxidase inhibitors in plant samples using different approaches of the biochromatography
Grantee:Marili Villa Nova Rodrigues
Support Opportunities: Regular Research Grants