LC-MS untargeted approach showed that methyl jasmonate application on Vitis labrusca L. grapes increases phenolics at subtropical Brazilian regions

Autor(es)

Moro, Lais ^[1] ; Da Ros, Alessio ^[2] ; da Mota, Renata Vieira ^[3] ; Purgatto, Eduardo ^[1] ; Mattivi, Fulvio ^{[2, 4]} ; Arapitsas, Panagiotis ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Deptartment Food Sci & Expt Nutr, FORC Food Res Ctr, Sao Paulo - Brazil

^[2] Res & Innovat Ctr Fdn Edmund Mach, Dept Food Qual & Nutr, San Michele All Adige - Italy

^[3] Empresa Pesquisa Agr Minas Gerais EPAMIG, Nucleo Tecnol Uva & Vinho, Caldas, MG - Brazil

^[4] Univ Trento, Ctr Agr Food Environm, Trento - Italy

• Tipo de documento: Artigo Científico
• Fonte: METABOLOMICS; v. 16, n. 2 JAN 23 2020.
• Citações Web of Science: 0

Resumo

Introduction Vitis labrusca L. grapes are largely cultivated in Brazil, but the tropical climate negatively affects the phenols content, especially anthocyanin. According to the projections of the incoming climatic changes, the climate of several viticulture zone might change to tropical. Therefore, researches are focusing on increasing grape phenols content; with methyl jasmonate application (MeJa) is considered a good alternative. Objectives The aim was to investigate with an untargeted approach the metabolic changes caused by the MeJa pre-harvest application on two Vitis labrusca L. cultivars grapes, both of them grown in two Brazilian regions. Methods Isabel Precoce and Concord grapes cultivated under subtropical climate, in the south and southeast of Brazil, received MeJa pre-harvest treatment. Grape metabolome was extracted and analyzed with a MS based metabolomics protocol by UPLC-HRMS-QTOF. Results Unsupervised data analysis revealed a clear separation between the two regions and the two cultivars, while supervised data analysis revealed biomarkers between the MeJa treatment group and the control group. Among the metabolites positively affected by MeJa were (a) flavonoids with a high degree of methylation at the B-ring (malvidin and peonidin derivatives and isorhamentin) for Isabel Precoce grapes; (b) glucosides of hydroxycinnamates, gallocatechin, epigallocatechin and cis-piceid for Concord grapes; and (c) hydroxycinnamates esters with tartaric acid, and procyanidins for the Southeast region grapes. Conclusion These results suggest that MeJa can be used as elicitor to secondary metabolism in grapes grown even under subtropical climate, affecting phenolic biosynthesis. (AU)

• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/08423-6 - Aplicação de metil jasmonato em uvas (Vitis labrusca) visando enriquecimento dos frutos quanto a compostos fenólicos (flavonóides e estilbenos)
• Beneficiário: Laís Moro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/20413-9 - Aplicação de metil jasmonato em uvas (Vitis labrusca) visando enriquecimento dos frutos quanto a compostos fenólicos (flavonóides e estilbenos)
• Beneficiário: Laís Moro
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado