Time-related immunomodulation by stressors and corticosterone transdermal application in toads

Autor(es)

Monteiro Titon, Stefanny Christie ^[1] ; Titon Jr, Braz ; Giorgi Barsotti, Adriana Maria ^[2] ; Gomes, Fernando Ribeiro ^[2] ; Assis, Vania Regina ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, SP - Brazil

^[2] Titon Jr, Jr., Braz, Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 9 SEP 20 2019.
• Citações Web of Science: 1

Resumo

Immune responses have been mostly studied at a specific time in anuran species. However, time-changes related to immunomodulation associated with glucocorticoid (GC) alterations following stressors and GC treatment are complex. The present study describes time-related changes in immune response and corticosterone (CORT) plasma levels following restraint challenge, short, mid and long-term captivity, and CORT exogenous administration by transdermal application (TA) in Rhinella ornata toads. We observed increased neutrophil: lymphocyte ratios after restraint challenge and CORT TA, without changes following short and mid-term captivity. Plasma bacterial killing ability was sustained in all treatments, except long-term captivity, with decreased values after 90 days under such conditions. Phagocytic activity of peritoneal cells increased after mid-term captivity, and the phytohemagglutinin swelling response was impaired in those animals treated with CORT TA for 20 consecutive days. Plasma CORT levels increased or were sustained after restraint challenge (depending on initial values), decreased following mid and long-term captivity (for those animals showing high CORT in the field) and increased after 20 days of CORT TA. By performing assessments of time-changes in immune processes and CORT plasma levels in R. ornata, we demonstrate immuno-enhancing effects following restraint, short and mid-term stressors, while long-term stressors and CORT TA promoted immunosuppression in these toads. (AU)

• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 11/10560-8 - Níveis plasmáticos de corticosterona, testosterona e imunocompetência em Bufonídeos.
• Beneficiário: Vania Regina de Assis
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/05024-3 - Estresse hídrico e resposta imune em sapos (Rhinella ornata) habitando fragmentos de Floresta Atlântica
• Beneficiário: Adriana Maria Giorgi Barsotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado