Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations

Autor(es)

Aguiar, Weslei da Silva ^{[1, 2]} ; Galizio, Nathalia da Costa ^{[1, 2]} ; Serino-Silva, Caroline ^{[1, 2]} ; Sant'Anna, Savio Stefanini ^[1] ; Grego, Kathleen Fernandes ^[1] ; Tashima, Alexandre Keiji ^[3] ; Nishiduka, Erika Sayuri ^[3] ; de Morais-Zani, Karen ^{[1, 2]} ; Tanaka-Azevedo, Anita Mitico ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Herpetol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 9 SEP 12 2019.
• Citações Web of Science: 0

Resumo

Snake venoms are complex protein mixtures with different biological activities that can act in both their preys and human victims. Many of these proteins play a role in prey capture and in the digestive process of these animals. It is known that some snakes are resistant to the toxicity of their own venom by mechanisms not yet fully elucidated. However, it was observed in the Laboratory of Herpetology of Instituto Butantan that some Bothrops moojeni individuals injured by the same snake species showed mortalities caused by envenoming effects. This study analyzed the biochemical composition of 13 venom and plasma samples from Bothrops moojeni specimens to assess differences in their protein composition. Application of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed distinct venom protein profiles, but very homogeneous plasma profiles. Western Blotting (WB) was performed with plasma samples, which were submitted to incubation with the respective venom. Some individuals showed an immunorecognized band zone around 25 kDa, indicating interaction between the same individual plasma and venom proteins. Crossed-WB assay using non-self-plasma and venom showed that this variability is due to venom protein composition instead of plasma composition. These venoms presented higher caseinolytic, collagenolytic and coagulant activities than the venoms without these regions recognized by WB. Mass spectrometry analyses performed on two individuals revealed that these individuals present, in addition to higher protein concentrations, other exclusive proteins in their composition. When these same two samples were tested in vivo, the results also showed higher lethality in these venoms, but lower hemorrhagic activity than in the venoms without these regions recognized by WB. In conclusion, some Bothrops moojeni specimens differ in venom composition, which may have implications in envenomation. Moreover, the high individual venom variability found in this species demonstrates the importance to work with individual analyses in studies involving intraspecific venom variability and venom evolution. (AU)

• Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém-chegadas da natureza
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
• Beneficiário: Alexandre Keiji Tashima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/20651-0 - Análise da variabilidade ontogenética e sazonal do veneno da serpente Bothrops jararacussu (Serpentes: Viperidae; Lacerda 1884)
• Beneficiário: Weslei da Silva Aguiar
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/14724-4 - Caracterização proteômica e funcional do veneno de duas espécies de serpentes do gênero Bothrops submetidas à mudança de dieta
• Beneficiário: Nathália da Costa Galizio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular