Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity

Autor(es)

Silva, L. N. ^{[1, 2]} ; Rigo, V, G. ; Silva, D. B. ^[3] ; Carollo, C. A. ^[3] ; Trentin, D. S. ^[4] ; Silva, V, M. ; Tasca, T. ^[5] ; Macedo, A. J. ^{[1, 5, 6]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, Porto Alegre, RS - Brazil

^[2] Univ Fed Rio Grande do Sul, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil

^[3] Univ Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LaPNEM, Campo Grande, MS - Brazil

^[4] Univ Fed Ciencias Saude Porto Alegre, Dept Ciencias Basicas Saude, Porto Alegre, RS - Brazil

^[5] Rigo, G., V, Univ Fed Rio Grande do Sul, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil

^[6] Silva, M., V, Univ Fed Pernambuco, Dept Bioquim, Recife, PE, Brazil.Silva, L. N., Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 134, AUG 2020.
• Citações Web of Science: 0

Resumo

Poincianella (Caesalpinia) microphylla fractions were analyzed by LC-DAD-MS and by statistical tools to determine the possible bioactive compounds against T. vaginalis. Tannins enriched-fractions toxicity (M5 and M10) were analyzed in in vitro against human red cells and in in vivo model of Galleria mellonella. Twenty-six compounds were detected from the P. microphylla fractions. The main compounds identified were hydrolyzable tannins (gallotanins and ellagitannis), such as O-digalloyl hexoside, O-digalloyl HHDP -hexoside, tri-O-galloyl HHDP-hexoside, O-galloyl HHDP-DHHDP-hexoside and their isomers. In addition, procyanidin dimer, epicatechin, ellagic acid and O-(digalloyl) quinic acid were also identified. Based on univariate statistical analyses, stronger correlations with the anti-T. vaginalis properties were observed for the compounds 7 (O-galloyl HHDP-DHHDP-hexoside - Geraniin isomer), 3 (O-digalloyl HHDP -hexoside - mallotinic acid), 2 (O-digalloyl) quinic acid), 1 (O-digalloyl hexoside) and 9 (unknown). Tannins enriched-fractions (M5 and M10) presented anti-Trichomonas activity (IC50 70.41 mu g/mL and 142.1 mu g/mL, respectively) and no toxicity in the in vivo model of G. mellonella. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying anti-trichomonal activity encompass a complex trait. (AU)

• Processo FAPESP: 12/18031-7 - Aplicação de MALDI-MS/MS na análise da vicenina-2 e correlatos: da compreensão dos processos ionizantes à geração de imagens
• Beneficiário: Denise Brentan da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/54098-6 - EMU: aquisição de um espectrômetro de massas para geração de imagens na Central de Espectrometria de Massas (mass-facility) da FCFRP-USP: estudos de localização molecular de substâncias biologicamente ativas
• Beneficiário: Norberto Peporine Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários