| Full text | |
| Author(s): |
Silva, L. N.
[1, 2]
;
Rigo, V, G.
;
Silva, D. B.
[3]
;
Carollo, C. A.
[3]
;
Trentin, D. S.
[4]
;
Silva, V, M.
;
Tasca, T.
[5]
;
Macedo, A. J.
[1, 5, 6]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, Porto Alegre, RS - Brazil
[2] Univ Fed Rio Grande do Sul, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil
[3] Univ Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LaPNEM, Campo Grande, MS - Brazil
[4] Univ Fed Ciencias Saude Porto Alegre, Dept Ciencias Basicas Saude, Porto Alegre, RS - Brazil
[5] Rigo, G., V, Univ Fed Rio Grande do Sul, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil
[6] Silva, M., V, Univ Fed Pernambuco, Dept Bioquim, Recife, PE, Brazil.Silva, L. N., Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, Porto Alegre, RS - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Food Research International; v. 134, AUG 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Poincianella (Caesalpinia) microphylla fractions were analyzed by LC-DAD-MS and by statistical tools to determine the possible bioactive compounds against T. vaginalis. Tannins enriched-fractions toxicity (M5 and M10) were analyzed in in vitro against human red cells and in in vivo model of Galleria mellonella. Twenty-six compounds were detected from the P. microphylla fractions. The main compounds identified were hydrolyzable tannins (gallotanins and ellagitannis), such as O-digalloyl hexoside, O-digalloyl HHDP -hexoside, tri-O-galloyl HHDP-hexoside, O-galloyl HHDP-DHHDP-hexoside and their isomers. In addition, procyanidin dimer, epicatechin, ellagic acid and O-(digalloyl) quinic acid were also identified. Based on univariate statistical analyses, stronger correlations with the anti-T. vaginalis properties were observed for the compounds 7 (O-galloyl HHDP-DHHDP-hexoside - Geraniin isomer), 3 (O-digalloyl HHDP -hexoside - mallotinic acid), 2 (O-digalloyl) quinic acid), 1 (O-digalloyl hexoside) and 9 (unknown). Tannins enriched-fractions (M5 and M10) presented anti-Trichomonas activity (IC50 70.41 mu g/mL and 142.1 mu g/mL, respectively) and no toxicity in the in vivo model of G. mellonella. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying anti-trichomonal activity encompass a complex trait. (AU) | |
| FAPESP's process: | 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images |
| Grantee: | Denise Brentan da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances |
| Grantee: | Norberto Peporine Lopes |
| Support Opportunities: | Multi-user Equipment Program |