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Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances

Grant number: 09/54098-6
Support Opportunities:Multi-user Equipment Program
Duration: October 01, 2010 - September 30, 2012
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Norberto Peporine Lopes
Grantee:Norberto Peporine Lopes
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment: Tipo de Equipamento Multiusuário não informado
Manufacturer: Fabricante não informado
Model: Modelo não informado


Nearly five years ago, we obtained, with the help of FAPESP, a high-resolution electrospray ionization mass spectrometer as part of a Thematic Project. On that occasion, Prof. José Fernando Perez, who was then the scientific director of FAPESP, suggested that we attempt to create a facility system such as those existing in more highly developed countries. On that basis, we created, within the USP Ribeirão Preto School of Pharmaceutical Sciences, a system of shared use, designated the Mass Spectrometry Center. Today, the equipment, at six years of age, is in operation seven days a week year round, and provides a large number of researchers throughout the state with access to high-resolution data. In recent years, it was possible to expand that access to include more than 29 federal universities, covering the entire country. This was only possible due to planning and to the training of a specialist dedicated exclusively to the mass spectrometry facility. In the current proposal, we intend to expand this center, with the purchase of imaging equipment that is as yet unavailable in the state of Sao Paulo: the maldi-tof-tof imaging system. As can be seen in the introduction of this proposal, the capacity of this equipment will bring advancements in various fields of research. We opted for a laser with greater power and resolution, in order to provide greater support to projects in the medical field, as well as for detection in the tissues of insects, thereby meeting the needs of biologists. The expectation of identifying secondary metabolites in plant tissues and the possible correlation with their effects might provide a qualitative leap in research conducted in the state. For the biochemists who are already using normal maldi, access to such equipment will allow them to “photograph” areas of study and propose a number of mechanisms of action. As can be seen, this is perhaps one of the most universal types of equipment, and we therefore elected to gather a group of researchers from various institutions to demonstrate that this machine is suited to use in different areas and applications. (AU)

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Scientific publications (16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, L. N.; RIGO, V, G.; SILVA, D. B.; CAROLLO, C. A.; TRENTIN, D. S.; SILVA, V, M.; TASCA, T.; MACEDO, A. J.. Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity. Food Research International, v. 134, . (12/18031-7, 09/54098-6)
VIEIRA, PATRICIA DE BRUM; FEIJO SILVA, NICOLAS LUIZ; SILVA, DENISE BRENTAN; LOPES, NORBERTO PEPORINE; DA SILVA, ALEXANDRE GOMES; DA SILVA, MARCIA VANUSA; BASTIDA, JAUME; MACEDO, ALEXANDRE JOSE; TASCA, TIANA. The Caatinga endemic Manilkara rufula possesses remarkable activity against Trichomonas vaginalis and Tritrichomonas foetus. Experimental Parasitology, v. 173, p. 18-28, . (12/18031-7, 09/54098-6)
MENEGATTI, CARLA; DA PAIXAO MELO, WEILAN GOMES; CARRAO, DANIEL BLASCKE; MORAES DE OLIVEIRA, ANDERSON RODRIGO; DO NASCIMENTO, FABIO SANTOS; LOPES, NORBERTO PEPORINE; PUPO, MONICA TALLARICO. Paenibacillus polymyxa Associated with the Stingless Bee Melipona scutellaris Produces Antimicrobial Compounds against Entomopathogens. Journal of Chemical Ecology, v. 44, n. 12, p. 1158-1169, . (09/54098-6, 14/17620-4, 13/50954-0, 14/50265-3, 15/01001-6)
VIEIRA, PATRCIA DE BRUM; FEIJO SILVA, NICOLAS LUIZ; SANTOS DA SILVA, GLORIA NARJARA; SILVA, DENISE BRENTAN; LOPES, NORBERTO PEPORINE; BAGGIO GNOATTO, SIMONE CRISTINA; DA SILVA, MARCIA VANUSA; MACEDO, ALEXANDRE JOSE; BASTIDA, JAUME; TASCA, TIANA. Caatinga plants: Natural and semi-synthetic compounds potentially active against Trichomonas vaginalis. Bioorganic & Medicinal Chemistry Letters, v. 26, n. 9, p. 2229-2236, . (12/18031-7, 09/54098-6)
SILVA, DENISE B.; LOPES, NORBERTO P.. MALDI-MS of flavonoids: a systematic investigation of ionization and in-source dissociation mechanisms. Journal of Mass Spectrometry, v. 50, n. 1, p. 182-190, . (12/18031-7, 09/54098-6)
SILVA, DENISE BRENTAN; ASCHENBRENNER, ANNA-KATHARINA; LOPES, NORBERTO PEPORINE; SPRING, OTMAR. Direct Analyses of Secondary Metabolites by Mass Spectrometry Imaging (MSI) from Sunflower (Helianthus annuus L.) Trichomes. Molecules, v. 22, n. 5, . (14/50265-3, 09/54098-6)
LACHI-SILVA, LARISSA; SY, SHERWIN K. B.; VOELKNER, ALEXANDER; BARRETO DE SOUSA, JOAO PAULO; LOPES, JOAO LUIS C.; SILVA, DENISE B.; LOPES, NORBERTO P.; KIMURA, ELZA; DERENDORF, HARTMUT; DINIZ, ANDREA. Simultaneous Characterization of Intravenous and Oral Pharmacokinetics of Lychnopholide in Rats by Transit Compartment Model. Planta Medica, v. 81, n. 12-13, p. 1121-1127, . (14/50265-3, 12/18031-7, 09/54098-6)
VON BOROWSKI, RAFAEL GOMES; BARROS, MURIEL PRIMON; DA SILVA, DENISE BRENTAN; LOPES, NORBERTO PEPORINE; ZIMMER, KARINE RIGON; STAATS, CHARLEY CHRISTIAN; DE OLIVEIRA, CRISTIANE BERNARDES; GIUDICE, EMMANUEL; GILLET, REYNALD; MACEDO, ALEXANDRE JOSE; et al. Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation. International Journal of Pharmaceutics, v. 574, . (09/54098-6)
PINA, EDIEIDIA S.; SILVA, DENISE B.; TEIXEIRA, SIMONE P.; COPPEDE, JULIANA S.; FURLAN, MAYSA; FRANCA, SUZELEI C.; LOPES, NORBERTO P.; PEREIRA, ANA MARIA S.; LOPES, ADRIANA A.. Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging. SCIENTIFIC REPORTS, v. 6, . (09/54098-6, 14/50265-3, 12/18031-7, 13/07349-9, 15/26066-3, 10/18132-2)
LACHI-SILVA, LARISSA; BARRETO SOUSA, JOAO PAULO; MONTANHA, MAIARA CAMOTTI; SY, SHERWIN K. B.; CALLEGARI LOPES, JOAO LUIS; SILVA, DENISE BRENTAN; LOPES, NORBERTO PEPORINE; DINIZ, ANDREA; KIMURA, ELZA. Rapid and efficient method for the quantification of lychnopholide in rat plasma by liquid chromatography-tandem mass spectrometry for pharmacokinetic application. BIOMEDICAL CHROMATOGRAPHY, v. 30, n. 7, p. 1092-1096, . (12/18031-7, 09/54098-6)
ERNST, MADELEINE; SILVA, DENISE B.; SILVA, RICARDO; MONGE, MARCELO; SEMIR, JOAO; VENCIO, RICARDO Z. N.; LOPES, NORBERTO P.. A metabolomic protocol for plant systematics by matrix-assisted laser-desorption/ionization time-of flight mass spectrometry. Analytica Chimica Acta, v. 859, p. 46-58, . (11/05215-0, 10/14926-4, 12/18031-7, 09/54098-6)
TRENTIN, DANIELLE S.; SILVA, DENISE B.; FRASSON, AMANDA P.; RZHEPISHEVSKA, OLENA; DA SILVA, MARCIA V.; PULCINI, ELINOR DE L.; JAMES, GARTH; SOARES, GABRIEL V.; TASCA, TIANA; RAMSTEDT, MADELEINE; et al. Natural Green Coating Inhibits Adhesion of Clinically Important Bacteria. SCIENTIFIC REPORTS, v. 5, . (14/50265-3, 12/18031-7, 09/54098-6)
PAVARINI, DANIEL P.; DA SILVA, DENISE B.; CAROLLO, CARLOS A.; PORTELLA, AMANDA P. F.; LATANSIO-AIDAR, SABRINA R.; CAVALIN, PEDRO O.; OLIVEIRA, VIVIANE C.; ROSADO, BRUNO H. P.; AIDAR, MARCOS P. M.; BOLZANI, VANDERLAN S.; et al. Application of MALDI-MS analysis of Rainforest chemodiversity: a keystone for biodiversity conservation and sustainable use. Journal of Mass Spectrometry, v. 47, n. 11, p. 1482-1485, . (03/12595-7, 03/02176-7, 10/50811-7, 09/54098-6)
SILVA, DENISE BRENTAN; CASANOVA TURATTI, IZABEL CRISTINA; GOUVEIA, DAYANA RUBIO; ERNST, MADELEINE; TEIXEIRA, SIMONE PADUA; LOPES, NORBERTO PEPORINE. Mass Spectrometry of Flavonoid Vicenin-2, Based Sunlight Barriers in Lychnophora species. SCIENTIFIC REPORTS, v. 4, . (12/18031-7, 10/50811-7, 09/54098-6)
ERNST, MADELEINE; SILVA, DENISE BRENTAN; SILVA, RICARDO ROBERTO; VENCIO, RICARDO Z. N.; LOPES, NORBERTO PEPORINE. Mass spectrometry in plant metabolomics strategies: from analytical platforms to data acquisition and processing. NATURAL PRODUCT REPORTS, v. 31, n. 6, p. 784-806, . (11/05215-0, 10/14926-4, 12/18031-7, 09/54098-6)
NUNES, TULIO M.; MATEUS, SIDNEI; FAVARIS, ARODI P.; AMARAL, MONICA F. Z. J.; VON ZUBEN, LUCAS G.; CLOSOSKI, GIULIANO C.; BENTO, JOSE M. S.; OLDROYD, BENJAMIN P.; SILVA, RICARDO; ZUCCHI, RONALDO; et al. Queen signals in a stingless bee: suppression of worker ovary activation and spatial distribution of active compounds. SCIENTIFIC REPORTS, v. 4, . (09/54098-6, 14/20302-4, 12/18031-7, 11/22991-3, 14/01884-2)

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