Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis

• Grant number: 09/54040-8
• Support type: Multi-user Equipment Program
• Duration: September 01, 2010 - March 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal researcher: Emanuel Carrilho
• Grantee: Emanuel Carrilho
• Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The equipment requested in this proposal is a highresolution mass spectrometer that works in tandem (MS-MS) in order to carry out multiple fragmentation stages. The equipment consists of a quadrupole linear ion trap (linear trap quadrupole, LTQ) coupled to an “Orbitrap” spectrometer. The LTQ-Orbitrap Velos is capable of providing the high precision necessary for the structural analysis of small molecules; rapid, diverse fragmentation processes for the sequencing of proteins and peptides; and analytical sensitivity for the characterization of trace metabolites, pollutants, and drugs in a wide variety of matrices. The incorporation of this equipment at the Instrumental Chemical Analysis Center of the USP São Carlos Chemistry Institute will make it possible to obtain unprecedented results in terms of the following: the structural analysis of organic compounds of low molecular mass, originating from total synthesis, enzymatic synthesis, redox processes, plant extracts, venoms, or microorganisms; the sequencing of proteins in proteomic, clinical, and biomarker studies, as well as in structural studies, functional studies, and studies of post-translational modifications, in vivo and in vitro, by heterologous protein expression; the analytical detectability of endogenous and exogenous compounds in complex matrices, such as environmental matrices, as well as those in food and beverages; and the development of human resources that are highly qualified to conduct research using mass spectrometry. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, TIAGO M. F.; CARRILHO, EMANUEL; GALINARO, CARLOS A.; CABRAL, FERNANDA J.; ALLEGRETTI, SILMARA M. Biomphalaria glabrata infected with Angiostrongylus cantonensis: Proteomic changes in the snail host. Acta Tropica, v. 212, DEC 2020. Web of Science Citations: 0.

CAIUBY, CLARICE A. D.; DE JESUS, MATHEUS P.; BURTOLOSO, ANTONIO C. B. alpha-Imino Iridium Carbenes from Imidoyl Sulfoxonium Ylides: Application in the One-Step Synthesis of Indoles. Journal of Organic Chemistry, v. 85, n. 11, p. 7433-7445, JUN 5 2020. Web of Science Citations: 0.

GALLO, RAFAEL D. C.; MOMO, PATRICIA B.; DAY, DAVID P.; BURTOLOSO, ANTONIO C. B. Catalytic Friedel-Crafts Alkylation of Electron Rich Aromatic Derivatives with alpha-Aryl Diazoacetates Mediated by Bronsted Acids. ORGANIC LETTERS, v. 22, n. 6, p. 2339-2343, MAR 20 2020. Web of Science Citations: 0.

FERNANDES MENDES, TIAGO MANUEL; CARRILHO, EMANUEL; FONSECA SIEUVE AFONSO, ANA JULIA PINTO; GALINARO, CARLOS ALEXANDRE; CABRAL, FERNANDA JANKU; ALLEGRETTI, SILMARA MARQUES. Proteomic, metabolic and immunological changes in Biomphalaria glabrata infected with Schistosoma mansoni. International Journal for Parasitology, v. 49, n. 13-14, p. 1049-1060, DEC 2019. Web of Science Citations: 0.

WACHTER, NAIHARA; AQUINO, JOSE MARIO; DENADAI, MARINA; BARREIRO, JULIANA C.; SILVA, ADILSON JOSE; CASS, QUEZIA B.; BOCCHI, NERILSO; ROCHA-FILHO, ROMEU C. Electrochemical degradation of the antibiotic ciprofloxacin in a flow reactor using distinct BDD anodes: Reaction kinetics, identification and toxicity of the degradation products. Chemosphere, v. 234, p. 461-470, NOV 2019. Web of Science Citations: 0.

WACHTER, NAIHARA; AQUINO, JOSE M.; DENADAI, MARINA; BARREIRO, JULIANA C.; SILVA, ADILSON J.; CASS, QUEZIA B.; ROCHA-FILHO, ROMEU C.; BOCCHI, NERILSO. Optimization of the electrochemical degradation process of the antibiotic ciprofloxacin using a double-sided beta-PbO2 anode in a flow reactor: kinetics, identification of oxidation intermediates and toxicity evaluation. Environmental Science and Pollution Research, v. 26, n. 5, p. 4438-4449, FEB 2019. Web of Science Citations: 6.

FREIRE, VITOR F.; SILVA, GABRIELA R.; YARIWAKE, JANETE H. Targeted-Analysis of beta-Carboline Alkaloids in Passionfruit ({''}Maracuja{''}) by SBSE(PDMS)-LC/Flu and UHPLC-MS. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. 775-781, APR 2018. Web of Science Citations: 1.

ZAWADZKI, A.; ALLOO, C.; GROSSI, A. B.; DO NASCIMENTO, E. S. P.; ALMEIDA, L. C.; BOGUSZ JUNIOR, S.; SKIBSTED, L. H.; CARDOSO, D. R. Effect of hop beta-acids as dietary supplement for broiler chickens on meat composition and redox stability. Food Research International, v. 105, p. 210-220, MAR 2018. Web of Science Citations: 2.

MESSIAS MIRANDA, BEATRIZ NOGUEIRA; FOTORAN, WESLEY LUZETTI; CANDURI, FERNANDA; FERREIRA SOUZA, DULCE HELENA; WUNDERLICH, GERHARD; CARRILHO, EMANUEL. Heterologous expression of Homo sapiens alpha-folate receptors in E. coli by fusion with a trigger factor for enhanced solubilization. Protein Expression and Purification, v. 142, p. 75-80, FEB 2018. Web of Science Citations: 1.

VÍTOR F. FREIRE; GABRIELA R. SILVA; JANETE H. YARIWAKE. Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. -, Abr. 2018.

DE ZAWADZKI, ANDRESSA; ARRIVETTI, LEANDRO O. R.; VIDAL, MARLLIA P.; CATAI, JONATAN R.; NASSU, RENATA T.; TULLIO, RYMER R.; BERNDT, ALEXANDRE; OLIVEIRA, CLAYTON R.; FERREIRA, ANTONIO G.; NEVES-JUNIOR, LUIZ F.; COLNAGO, LUIZ A.; SKIBSTED, LEIF H.; CARDOSO, DANIEL R. Mate extract as feed additive for improvement of beef quality. Food Research International, v. 99, n. 1, p. 336-347, SEP 2017. Web of Science Citations: 7.

PESTANA, CEZAR RANGEL; URBACZEK, ANA CAROLINA; ALBERICI, JULIANA VIEIRA; RODRIGUES, GERSON JHONATAN; CARRILHO, EMANUEL. Metabolic profiling of human endothelial cells during autophagy assessed in a biomimetic microfluidic device model. Life Sciences, v. 172, p. 42-47, MAR 1 2017. Web of Science Citations: 3.

MELO, PRISCILLA SIQUEIRA; RODRIGUES ARRIVETTI, LEANDRO DE OLIVEIRA; DE ALENCAR, SEVERINO MATIAS; SKIBSTED, LEIF H. Antioxidative and prooxidative effects in food lipids and synergism with alpha-tocopherol of acai seed extracts and grape rachis extracts. Food Chemistry, v. 213, p. 440-449, DEC 15 2016. Web of Science Citations: 14.

MENEGOCI EUGENIO, PATRICIA DE FATIMA; ASSUNCAO, NILSON ANTONIO; SCIANDRA, FRANCESCA; AQUINO, ADRIANO; BRANCACCIO, ANDREA; CARRILHO, EMANUEL. Quantification, 2DE analysis and identification of enriched glycosylated proteins from mouse muscles: Difficulties and alternatives. ELECTROPHORESIS, v. 37, n. 2, p. 321-334, JAN 2016. Web of Science Citations: 0.

SANTOS, WILLY G.; PINA, JOAO; BURROWS, HUGH D.; FORBES, MALCOLM D. E.; CARDOSO, DANIEL R. New insight into the photophysics and reactivity of trigonal and tetrahedral arylboron compounds. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 15, n. 9, p. 1124-1137, 2016. Web of Science Citations: 4.

SANTOS, WILLY G.; SCURACHIO, REGINA S.; CARDOSO, DANIEL R. Photochemical behavior of Safranine-Riboflavin complex in the degradation of folic acid. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, v. 293, p. 32-39, NOV 1 2014. Web of Science Citations: 9.

TOMAZELLI COLTRO, WENDELL KARLOS; CHENG, CHAO-MIN; CARRILHO, EMANUEL; DE JESUS, DOSIL PEREIRA. Recent advances in low-cost microfluidic platforms for diagnostic applications. ELECTROPHORESIS, v. 35, n. 16, SI, p. 2309-2324, AUG 2014. Web of Science Citations: 85.

BERNARDIM, BARBARA; BURTOLOSO, ANTONIO C. B. A two-step synthesis of the bioprotective agent JP4-039 from N-Boc-L-leucinal. Tetrahedron, v. 70, n. 20, p. 3291-3296, MAY 20 2014. Web of Science Citations: 6.

SANTOS, WILLY G.; CARDOSO, DANIEL R.; ARRIVETTI, LEANDRO DE O. R.; SCHMITT, CARLA C.; NEUMANN, MIGUEL G. DFT, spectroscopic, and photoproduct study of 2-aminoethyldiphenylborinate and tetraphenyldiboroxane. JOURNAL OF ORGANOMETALLIC CHEMISTRY, v. 755, p. 125-133, APR 1 2014. Web of Science Citations: 2.

ALI, AKBAR; CORREA, ARLENE G.; ALVES, DIEGO; ZUKERMAN-SCHPECTOR, JULIO; WESTERMANN, BERNHARD; FERREIRA, MARCO A. B.; PAIXAO, MARCIO W. An efficient one-pot strategy for the highly regioselective metal-free synthesis of 1,4-disubstituted-1,2,3-triazoles. CHEMICAL COMMUNICATIONS, v. 50, n. 80, p. 11926-11929, 2014. Web of Science Citations: 44.

SANGI, DIEGO P.; MONTEIRO, JULIA L.; VANZOLINI, KENIA L.; CASS, QUEZIA B.; PAIXAO, MARCIO W.; CORREA, ARLENE G. Microwave-Assisted Synthesis of N-Heterocycles and Their Evaluation Using an Acetylcholinesterase Immobilized Capillary Reactor. Journal of the Brazilian Chemical Society, v. 25, n. 5, p. 887-889, Maio 2014. Web of Science Citations: 11.

DE ALMEIDA, NATALIA E. C.; LUND, MARIANNE N.; ANDERSEN, MOGENS L.; CARDOSO, DANIEL R. Beer Thiol-Containing Compounds and Redox Stability: Kinetic Study of 1-Hydroxyethyl Radical Scavenging Ability. Journal of Agricultural and Food Chemistry, v. 61, n. 39, p. 9444-9452, OCT 2 2013. Web of Science Citations: 14.

ARRIVETTI, LEANDRO DE O. R.; SCURACHIO, REGINA S.; SANTOS, WILLY G.; PAPA, THIAGO B. R.; SKIBSTED, LEIF H.; CARDOSO, DANIEL R. Photooxidation of Other B-Vitamins as Sensitized by Riboflavin. Journal of Agricultural and Food Chemistry, v. 61, n. 31, p. 7615-7620, AUG 7 2013. Web of Science Citations: 4.

DE ALMEIDA, NATALIA E. C.; DO NASCIMENTO, EDUARDO S. P.; CARDOSO, DANIEL R. On the Reaction of Lupulones, Hops beta-Acids, with 1-Hydroxyethyl Radical. Journal of Agricultural and Food Chemistry, v. 60, n. 42, p. 10649-10656, OCT 24 2012. Web of Science Citations: 9.