Virulence Potential of a Multidrug-ResistantEscherichia coliStrain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

Autor(es):Mostrar menos -

Santos, Ana Carolina M. ^[1] ; Silva, Rosa M. ^[1] ; Valiatti, Tiago B. ^[1] ; Santos, Fernanda E. ^[1] ; Santos-Neto, Jose E. ^[1] ; Cayo, Rodrigo ^{[2, 3]} ; Streling, Ana P. ^[2] ; Nodari, Carolina S. ^[2] ; Gales, Ana C. ^[2] ; Nishiyama-Jr, Milton Y. ; Carvalho, Eneas ^[4] ; Gomes, Tania A. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Dept Microbiol Imunol & Parasitol DMIP, Disciplina Microbiol, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Dept Med, Lab Alerta, Disciplina Infectol, BR-04039032 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Biol CDB, Lab Imunol & Bacteriol LIB, Setor Biol Mol Microbi, BR-09972270 Diadema, SP - Brazil

^[4] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 8, n. 6 JUN 2020.
• Citações Web of Science: 0

Resumo

Escherichia coliEC121 is a multidrug-resistant (MDR) strain isolated from a bloodstream infection of an inpatient with persistent gastroenteritis and T-zone lymphoma that died due to septic shock. Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenicE. coli.Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. The genomic analysis showed that strain EC121 harbors more than 50 complete virulence genetic clusters. It also displays the capacity to adhere to a variety of epithelial cell lineages and invade T24 bladder cells, as well as the ability to form biofilms on abiotic surfaces, and survive the bactericidal serum complement activity. Additionally, EC121 was shown to be virulent in theGalleria mellonellamodel. Furthermore, EC121 is an MDR strain harboring 14 antimicrobial resistance genes, includingbla(CTX-M-2). Completing the scenario, it belongs to serotype O154:H25 and to sequence type 101-B1, which has been epidemiologically linked to extraintestinal infections as well as to antimicrobial resistance spread. This study withE. colistrain EC121 shows that clinical isolates considered opportunistic might be true pathogens that go underestimated. (AU)

• Processo FAPESP: 16/01656-5 - Epidemiologia Genômica de bactérias de interesse médico
• Beneficiário: Enéas de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21947-7 - Análise do genoma e do potencial de virulência de uma cepa de Escherichia coli uropatogênica (UPEC) portadora de marcadores de E. coli diarreiogênica (DEC)
• Beneficiário: Tiago Barcelos Valiatti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/00402-6 - Estudo temporal da variabilidade de fatores de virulência e grupos filogenéticos de amostras de Escherichia coli patogênica extra-intestinal (ExPEC) isoladas de bacteremia em um hospital de São Paulo: sua relação com a evolução de ExPEC
• Beneficiário: Rosa Maria Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/17353-7 - ANÁLISE COMPARATIVA DE CARACTERÍSTICAS DE VIRULÊNCIA DE Escherichia coli DIARREIOGÊNICA EM CEPAS DE E. coli ISOLADAS DE PACIENTES COM DIFERENTES TIPOS DE INFECÇÃO URINÁRIA
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular