Virulence Potential of a Multidrug-ResistantEscherichia coliStrain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

Author(s):Show less -

Santos, Ana Carolina M. ^[1] ; Silva, Rosa M. ^[1] ; Valiatti, Tiago B. ^[1] ; Santos, Fernanda E. ^[1] ; Santos-Neto, Jose E. ^[1] ; Cayo, Rodrigo ^{[2, 3]} ; Streling, Ana P. ^[2] ; Nodari, Carolina S. ^[2] ; Gales, Ana C. ^[2] ; Nishiyama-Jr, Milton Y. ; Carvalho, Eneas ^[4] ; Gomes, Tania A. T. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Dept Microbiol Imunol & Parasitol DMIP, Disciplina Microbiol, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Dept Med, Lab Alerta, Disciplina Infectol, BR-04039032 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Biol CDB, Lab Imunol & Bacteriol LIB, Setor Biol Mol Microbi, BR-09972270 Diadema, SP - Brazil

^[4] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: MICROORGANISMS; v. 8, n. 6 JUN 2020.
• Web of Science Citations: 0

Abstract

Escherichia coliEC121 is a multidrug-resistant (MDR) strain isolated from a bloodstream infection of an inpatient with persistent gastroenteritis and T-zone lymphoma that died due to septic shock. Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenicE. coli.Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. The genomic analysis showed that strain EC121 harbors more than 50 complete virulence genetic clusters. It also displays the capacity to adhere to a variety of epithelial cell lineages and invade T24 bladder cells, as well as the ability to form biofilms on abiotic surfaces, and survive the bactericidal serum complement activity. Additionally, EC121 was shown to be virulent in theGalleria mellonellamodel. Furthermore, EC121 is an MDR strain harboring 14 antimicrobial resistance genes, includingbla(CTX-M-2). Completing the scenario, it belongs to serotype O154:H25 and to sequence type 101-B1, which has been epidemiologically linked to extraintestinal infections as well as to antimicrobial resistance spread. This study withE. colistrain EC121 shows that clinical isolates considered opportunistic might be true pathogens that go underestimated. (AU)

• FAPESP's process: 16/01656-5 - Genomic Epidemiology of bacteria of medical interest
• Grantee: Enéas de Carvalho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/21947-7 - Analysis of the genome and virulence potential of a uropathogenic Escherichia coli (UPEC) strain carrying diarrheogenic E. coli (DEC) markers
• Grantee: Tiago Barcelos Valiatti
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/00402-6 - Temporal study of the variability of virulence factors and phylogenetic groups of extraintestinal pathogenic Escherichia coli (ExPEC) isolated from bacteremia in patients of one Hospital in Sao Paulo: its relationship with ExPEC evolution
• Grantee: Rosa Maria Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/17353-7 - COMPARATIVE ANALYSIS OF VIRULENCE CHARACTERISTICS OF DIARRHEIOGENIC Escherichia coli IN E. coli STRAINS ISOLATED FROM PATIENTS WITH DIFFERENT TYPES OF URINARY INFECTION
• Grantee: Tânia Aparecida Tardelli Gomes do Amaral
• Support Opportunities: Regular Research Grants