Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment

Autor(es)

Silva, Vinicius da Eira ^{[1, 2]} ; Painelli, Vitor de Salles ^{[1, 2]} ; Shinjo, Samuel Katsuyuki ^[2] ; Pereira, Wagner Ribeiro ^{[1, 2]} ; Cilli, Eduardo Maffud ^[3] ; Sale, Craig ^{[4, 5, 6]} ; Gualano, Bruno ^{[1, 2]} ; Otaduy, Maria Concepcion ^[7] ; Artioli, Guilherme Giannini ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Appl Physiol & Nutr Res Grp, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Div Rheumatol, Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Dept Biochem & Technol Chem, Ribeirao Preto, SP - Brazil

^[4] Nottingham Trent Univ, Sport Hlth & Performance Enhancement Res Ctr, Nottingham - England

^[5] Nottingham Trent Univ, Musculoskeletal Physiol Res Grp, Nottingham - England

^[6] Nottingham Trent Univ, Sch Sci & Technol, Nottingham - England

^[7] Univ Sao Paulo, Sch Med, Inst Radiol & Oncol LIM44, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAR 17 2020.
• Citações Web of Science: 1

Resumo

Carnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography - HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error. (AU)

• Processo FAPESP: 14/11948-8 - Vida sem carnosina: desenvolvimento e caracterização de um modelo de rato nocaute para estudo do papel fisiológico da carnosina e suas implicações para o exercício físico e metabolismo muscular
• Beneficiário: Guilherme Giannini Artioli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/04806-0 - Efeitos de 12 semanas de treinamento intermitente de alta intensidade sobre as concentrações intramusculares de carnosina
• Beneficiário: Vitor de Salles Painelli
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/14746-4 - Metabolismo da carnosina no músculo esquelético: um estudo de múltiplas abordagens
• Beneficiário: Bruno Gualano
• Modalidade de apoio: Auxílio à Pesquisa - Temático