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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment

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Author(s):
Silva, Vinicius da Eira [1, 2] ; Painelli, Vitor de Salles [1, 2] ; Shinjo, Samuel Katsuyuki [2] ; Pereira, Wagner Ribeiro [1, 2] ; Cilli, Eduardo Maffud [3] ; Sale, Craig [4, 5, 6] ; Gualano, Bruno [1, 2] ; Otaduy, Maria Concepcion [7] ; Artioli, Guilherme Giannini [1, 2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Appl Physiol & Nutr Res Grp, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Div Rheumatol, Sao Paulo, SP - Brazil
[3] Univ Estadual Paulista UNESP, Dept Biochem & Technol Chem, Ribeirao Preto, SP - Brazil
[4] Nottingham Trent Univ, Sport Hlth & Performance Enhancement Res Ctr, Nottingham - England
[5] Nottingham Trent Univ, Musculoskeletal Physiol Res Grp, Nottingham - England
[6] Nottingham Trent Univ, Sch Sci & Technol, Nottingham - England
[7] Univ Sao Paulo, Sch Med, Inst Radiol & Oncol LIM44, BR-01246903 Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 MAR 17 2020.
Web of Science Citations: 1
Abstract

Carnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography - HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error. (AU)

FAPESP's process: 13/04806-0 - Effects of 12 weeks of high-intensity intermittent training on intramuscular carnosine concentrations
Grantee:Vitor de Salles Painelli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/14746-4 - Carnosine metabolism in skeletal muscle: a multi-approach study
Grantee:Bruno Gualano
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/11948-8 - Life without carnosine: development and characterization of a KO rat model for studying the physiological role of carnosine and its implications to physical exercise and muscle metabolism
Grantee:Guilherme Giannini Artioli
Support type: Research Grants - Young Investigators Grants