Molecular investigation of Phryganella acropodia Hertwig et Lesser, 1874 (Arcellinida, Amoebozoa)

Autor(es)

Dumack, Kenneth ^[1] ; Goerzen, Diana ^[1] ; Gonzalez-Miguens, Ruben ^[2] ; Siemensma, Ferry ^[3] ; Lahr, Daniel J. G. ^[4] ; Lara, Enrique ^[2] ; Bonkowski, Michael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Cologne, Inst Zool, Terr Ecol, Zulpicher Str 47b, D-50674 Cologne - Germany

^[2] CSIC, Real Jardin Bot, Plaza Murillo 2, ES-28014 Madrid - Spain

^[3] Julianaweg 10, NL-1241VW Kortenhoef - Netherlands

^[4] Univ Sao Paulo, Dept Zool, Inst Biosci, Rua Matao Tv 14 101, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF PROTISTOLOGY; v. 75, AUG 2020.
• Citações Web of Science: 0

Resumo

Phryganella acropodia Hertwig and Lesser, 1874, is one of the most common and abundant testate amoeba species. It represents the type species of the genus Phryganella Pcnard, 1902, which in turn is the type genus for the suborder Phryganellina (Arcellinida) Bovee, 1985, but despite its taxonomic importance it was not yet analyzed with molecular methods. We established two cultures of putative Phryganella acropodia, designed Phryganellina-specific primers, amplified SSU rDNA data and subjected these sequences to phylogenetic analyses. Morphological and genetic differences were found between both strains. With SSU rDNA phylogenetic analyses we confirm that Phryganella acropodia branches with Phryganella paradoxa Pcnard, 1902 and Cryptodifflugia Penard, 1890 in the Phryganellina. We thus give further evidence that pseudopodia morphology in the Arcellinida is a character of high taxonomic value, as suggested by Bovee and Jung when erecting the suborder Phryganellina. Moreover, we provide evidence for cryptic diversity and for the first time confirm the existence of a naked life stage in Arcellinida by molecular means. (C) 2020 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 13/04585-3 - Decifrando as grandes tendências de evolução molecular e morfológica nos Amoebozoa
• Beneficiário: Daniel José Galafasse Lahr
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores