Generation of neural progenitor cells from porcine-induced pluripotent stem cells

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Machado, Lucas Simoes ^{[1, 2]} ; Pieri, Naira Caroline Godoy ^[3] ; Botigelli, Ramon Cesar ^[4] ; de Castro, Raquel Vasconcelos Guimaraes ^[5] ; de Souza, Aline Fernanda ^[1] ; Bridi, Alessandra ^[1] ; Lima, Marina Amaro ^{[1, 2]} ; Fantinato Neto, Paulo ^[1] ; Pessoa, Lais Vicari de Figueiredo ^[1] ; Martins, Simone Maria Massami Kitamura ^[6] ; De Andrade, Andre Furugen Cesar ^[3] ; Freude, Kristine Karla ^[7] ; Bressan, Fabiana Fernandes ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil

^[4] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil

^[5] Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal - Brazil

^[6] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga - Brazil

^[7] Univ Copenhagen, Dept Vet & Anim Sci, Copenhagen - Denmark

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 14, n. 12 NOV 2020.
• Citações Web of Science: 0

Resumo

In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research. (AU)

• Processo FAPESP: 17/13973-8 - Geração e caracterização funcional de células neurais derivadas de iPSCs de pacientes com doenças neurodegenerativas
• Beneficiário: Lucas Simões Machado
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 15/26818-5 - Investigação de mecanismos celulares e moleculares da aquisição da toti- e pluripotência induzida in vitro - modelo translacional
• Beneficiário: Fabiana Fernandes Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/02159-8 - Indução da pluripotência celular e diferenciação in vitro no modelo suíno como modelo translacional
• Beneficiário: Lucas Simões Machado
• Modalidade de apoio: Bolsas no Brasil - Mestrado