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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Generation of neural progenitor cells from porcine-induced pluripotent stem cells

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Author(s):
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Machado, Lucas Simoes [1, 2] ; Pieri, Naira Caroline Godoy [3] ; Botigelli, Ramon Cesar [4] ; de Castro, Raquel Vasconcelos Guimaraes [5] ; de Souza, Aline Fernanda [1] ; Bridi, Alessandra [1] ; Lima, Marina Amaro [1, 2] ; Fantinato Neto, Paulo [1] ; Pessoa, Lais Vicari de Figueiredo [1] ; Martins, Simone Maria Massami Kitamura [6] ; De Andrade, Andre Furugen Cesar [3] ; Freude, Kristine Karla [7] ; Bressan, Fabiana Fernandes [1, 2]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil
[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil
[4] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
[5] Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal - Brazil
[6] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga - Brazil
[7] Univ Copenhagen, Dept Vet & Anim Sci, Copenhagen - Denmark
Total Affiliations: 7
Document type: Journal article
Source: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 14, n. 12 NOV 2020.
Web of Science Citations: 0
Abstract

In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research. (AU)

FAPESP's process: 17/13973-8 - Generation and functional characterization of neuronal cells derived from iPSCs from patients with neurodegenerative diseases
Grantee:Lucas Simões Machado
Support type: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach
Grantee:Fabiana Fernandes Bressan
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 17/02159-8 - Induction of cell pluripotency and in vitro differentiation in the porcine model as a translational model
Grantee:Lucas Simões Machado
Support type: Scholarships in Brazil - Master