Induction of cell pluripotency and in vitro differentiation in the porcine model as a translational model

• Grant number: 17/02159-8
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2017
• Effective date (End): December 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Fabiana Fernandes Bressan
• Grantee: Lucas Simões Machado
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach, AP.JP
• Associated scholarship(s): 17/13973-8 - Generation and functional characterization of neuronal cells derived from iPSCs from patients with neurodegenerative diseases, BE.EP.MS

Abstract

In 2006, Takahashi and collaborators reported the induction to pluripotency of somatic cells (induced pluripotent stem cells, iPS). Since then, this technique has much been developed; many animal models have been created opening a new series of opportunities in research. They allow us the creation of models for human genetic diseases, for example, amyotrophic lateral sclerosis, autism, schizophrenia, Parkinson´s disease, Alzheimer´s disease and the enhancement of relevant characteristics in agriculture. The pig model is considered to have many advantages over the others. Initial methods of induction relied on an integrative system by use of viral vectors permanently altering the host cell´s genome. These methods can cause mutations that interfere with the generated cell´s function and residual transgene expression may alter the cell´s function, tumorigenicity, and differentiation. Alternative methods are desired for the safety that they bring for not being of an integrative nature. The episomal vector is a plasmid inserted of Epstein-Barr virus´ (EBV) EBNA1 gene and OriP sequence that allows it to replicate extrachromosomally and it is gradually and spontaneously eliminated from the cells. Acknowledging the benefits of the pig model to the different segments of research, biomedicine and agriculture, and the importance of more studies in the iPS cellular reprogramming, this project aims to generated porcine iPS by use of episomal vectors and differentiate those cells into neural progenitor cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, LUCAS SIMOES; PIERI, NAIRA CAROLINE GODOY; BOTIGELLI, RAMON CESAR; DE CASTRO, RAQUEL VASCONCELOS GUIMARAES; DE SOUZA, ALINE FERNANDA; BRIDI, ALESSANDRA; LIMA, MARINA AMARO; FANTINATO NETO, PAULO; PESSOA, LAIS VICARI DE FIGUEIREDO; MARTINS, SIMONE MARIA MASSAMI KITAMURA; et al. Generation of neural progenitor cells from porcine-induced pluripotent stem cells. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 14, n. 12, NOV 2020. (17/13973-8, 15/26818-5, 13/08135-2, 17/02159-8)

MACHADO, LUCAS SIMOES; RECCHIA, KAIANA; GODOY PIERI, NAIRA CAROLINE; BOTIGELLI, RAMON CESAR; GUIMARAES DE CASTRO, RAQUEL VASCONCELOS; CRUZ, JESSICA BRUNHARA; DE FIGUEIREDO PESSOA, LAIS VICARI; BRESSAN, FABIANA FERNANDES. Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs). JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 172, JUN 2021. (17/13973-8, 15/26818-5, 17/02159-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MACHADO, Lucas Simões. Induction of cell pluripotency and in vitro differentiation in swine as a translational model. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.