Generation and functional characterization of neuronal cells derived from iPSCs from patients with neurodegenerative diseases

• Grant number: 17/13973-8
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): January 05, 2018
• Effective date (End): July 04, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Fabiana Fernandes Bressan
• Grantee: Lucas Simões Machado
• Supervisor: Poul Hyttel
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Research place: University of Copenhagen, Frederiksberg, Denmark
• Associated to the scholarship: 17/02159-8 - Induction of cell pluripotency and in vitro differentiation in the porcine model as a translational model, BP.MS

Abstract

Induced pluripotent stem cells (iPSCs) are pluripotent cells derived from differentiated cells that have reacquired pluripotency through expression of specific transcription factors. The process is also referred to as cellular reprogramming. This unique method has enabled us to study diseases for which it was challenging to obtain patient samples. Due to the limitations of accessing brain samples from patients; directed differentiation of iPSC into neural subtypes affected by neurodegenerative diseases are of great value. This technology allowed us to create new in vitro models of neurodegenerative syndromes and diseases and moreover specific neural cell types that can be cultivated over long periods of time. Consequently, these techniques open up for new possibilities of regenerative medicine and drug screening platforms. We have previously successfully reprogrammed fibroblasts from human patients with Alzheimer´s Disease (AD) carrying mutations in the presenilin 1 gene (PSEN1), which is commonly mutated in familial forms of AD. In addition we have repaired the single nucleotide mutations in PSEN1 using the CRISPR/Cas9 system. Therefore, this project proposes to generate AD iPSCs-derived neurons and repaired iPSCs-derived neurons in order to compare their transcriptomic and proteomic profile.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, LUCAS SIMOES; RECCHIA, KAIANA; GODOY PIERI, NAIRA CAROLINE; BOTIGELLI, RAMON CESAR; GUIMARAES DE CASTRO, RAQUEL VASCONCELOS; CRUZ, JESSICA BRUNHARA; DE FIGUEIREDO PESSOA, LAIS VICARI; BRESSAN, FABIANA FERNANDES. Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs). JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 172, JUN 2021. (17/13973-8, 15/26818-5, 17/02159-8)

MACHADO, LUCAS SIMOES; PIERI, NAIRA CAROLINE GODOY; BOTIGELLI, RAMON CESAR; DE CASTRO, RAQUEL VASCONCELOS GUIMARAES; DE SOUZA, ALINE FERNANDA; BRIDI, ALESSANDRA; LIMA, MARINA AMARO; FANTINATO NETO, PAULO; PESSOA, LAIS VICARI DE FIGUEIREDO; MARTINS, SIMONE MARIA MASSAMI KITAMURA; et al. Generation of neural progenitor cells from porcine-induced pluripotent stem cells. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 14, n. 12, NOV 2020. (17/13973-8, 15/26818-5, 13/08135-2, 17/02159-8)