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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Telmisartan Prevents Alveolar Bone Loss by Decreasing the Expression of Osteoclasts Markers in Hypertensive Rats With Periodontal Disease

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Autor(es):
Brito, Victor Gustavo Balera [1, 2] ; Patrocinio, Mariana Sousa [1, 2] ; de Sousa, Maria Carolina Linjardi [1, 2] ; Barreto, Ayna Emanuelli Alves [1, 2] ; Frasnelli, Sabrina Cruz Tfaile [1, 2] ; Lara, Vanessa Soares [3] ; Santos, Carlos Ferreira [4] ; Oliveira, Sandra Helena Penha [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Multictr Postgrad Program Physiol Sci, Brazilian Soc Physiol, Aracatuba - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Bauru, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PHARMACOLOGY; v. 11, NOV 11 2020.
Citações Web of Science: 0
Resumo

Periodontal disease (PD) is a prevalent inflammatory disease with the most severe consequence being the loss of the alveolar bone and teeth. We therefore aimed to evaluate the effects of telmisartan (TELM), an angiotensin II type 1 receptor (Agtr1) antagonist, on the PD-induced alveolar bone loss, in Wistar (W) and Spontaneous Hypertensive Rats (SHRs). PD was induced by ligating the lower first molars with silk, and 10 mg/kg TELM was concomitantly administered for 15 days. The hemimandibles were subjected to microtomography, ELISA was used for detecting tumor necrosis factor (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), CXCL3, and CCL2, while qRT-PCR was used for analyzing expression of components of renin-angiotensin system (RAS) (Agt, Ace, Agt1r, Agt2r, Ace2, and Masr), and bone markers (Runx2, Osx, Catnb, Alp, Col1a1, Opn, Ocn, Bsp, Bmp2, Trap, Rank, Rankl, CtsK, Mmp-2, Mmp-9, and osteoclast-associated receptor (Oscar)). The SHR + PD group showed greater alveolar bone loss than the W + PD group, what was significantly inhibited by treatment with TELM, especially in the SHR group. Additionally, TELM reduced the production of TNF-alpha, IL-1 beta, and CXCL3 in the SHR group. The expression of Agt increased in the groups with PD, while Agtr2 reduced, and TELM reduced the expression of Agtr1 and increased the expression of Agtr2, in W and SHRs. PD did not induce major changes in the expression of bone formation markers, except for the expression of Alp, which decreased in the PD groups. The bone resorption markers expression, Mmp9, Ctsk, and Vtn, was higher in the SHR + PD group, compared to the respective control and W + PD group. However, TELM attenuated these changes and increased the expression of Runx2 and Alp. Our study suggested that TELM has a protective effect on the progression of PD, especially in hypertensive animals, as evaluated by the resorption of the lower alveolar bone. This can be partly explained by the modulation in the expression of Angiotensin II receptors (AT1R and AT2R), reduced production of inflammatory mediators, the reduced expression of resorption markers, and the increased expression of the bone formation markers. (AU)

Processo FAPESP: 17/02271-2 - O papel do Telmisartan na diferenciação osteoblástica em animais espontaneamente hipertensos
Beneficiário:Mariana Sousa Patrocinio
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 18/23676-3 - Participação dos microRNAs nas alterações ósseas locais induzidas pela doença periodontal e pelo sistema renina-angiotensina local em ratos espontaneamente hipertensos
Beneficiário:Victor Gustavo Balera Brito
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/05873-3 - Análise in vitro do papel dos mastócitos e do sistema renina-angiotensina sobre osteoblastos e osteoclastos de animais espontaneamente hipertensos
Beneficiário:Sabrina Cruz Tfaile Frasnelli
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/07095-8 - Papel dos mastócitos sobre a diferenciação osteogênica in vitro de células mesenquimais estromais da medula óssea de camundongos
Beneficiário:Maria Carolina Linjardi de Sousa
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
Beneficiário:Carlos Ferreira dos Santos
Linha de fomento: Auxílio à Pesquisa - Temático