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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Telmisartan Prevents Alveolar Bone Loss by Decreasing the Expression of Osteoclasts Markers in Hypertensive Rats With Periodontal Disease

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Author(s):
Brito, Victor Gustavo Balera [1, 2] ; Patrocinio, Mariana Sousa [1, 2] ; de Sousa, Maria Carolina Linjardi [1, 2] ; Barreto, Ayna Emanuelli Alves [1, 2] ; Frasnelli, Sabrina Cruz Tfaile [1, 2] ; Lara, Vanessa Soares [3] ; Santos, Carlos Ferreira [4] ; Oliveira, Sandra Helena Penha [1, 2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Multictr Postgrad Program Physiol Sci, Brazilian Soc Physiol, Aracatuba - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Bauru, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN PHARMACOLOGY; v. 11, NOV 11 2020.
Web of Science Citations: 0
Abstract

Periodontal disease (PD) is a prevalent inflammatory disease with the most severe consequence being the loss of the alveolar bone and teeth. We therefore aimed to evaluate the effects of telmisartan (TELM), an angiotensin II type 1 receptor (Agtr1) antagonist, on the PD-induced alveolar bone loss, in Wistar (W) and Spontaneous Hypertensive Rats (SHRs). PD was induced by ligating the lower first molars with silk, and 10 mg/kg TELM was concomitantly administered for 15 days. The hemimandibles were subjected to microtomography, ELISA was used for detecting tumor necrosis factor (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), CXCL3, and CCL2, while qRT-PCR was used for analyzing expression of components of renin-angiotensin system (RAS) (Agt, Ace, Agt1r, Agt2r, Ace2, and Masr), and bone markers (Runx2, Osx, Catnb, Alp, Col1a1, Opn, Ocn, Bsp, Bmp2, Trap, Rank, Rankl, CtsK, Mmp-2, Mmp-9, and osteoclast-associated receptor (Oscar)). The SHR + PD group showed greater alveolar bone loss than the W + PD group, what was significantly inhibited by treatment with TELM, especially in the SHR group. Additionally, TELM reduced the production of TNF-alpha, IL-1 beta, and CXCL3 in the SHR group. The expression of Agt increased in the groups with PD, while Agtr2 reduced, and TELM reduced the expression of Agtr1 and increased the expression of Agtr2, in W and SHRs. PD did not induce major changes in the expression of bone formation markers, except for the expression of Alp, which decreased in the PD groups. The bone resorption markers expression, Mmp9, Ctsk, and Vtn, was higher in the SHR + PD group, compared to the respective control and W + PD group. However, TELM attenuated these changes and increased the expression of Runx2 and Alp. Our study suggested that TELM has a protective effect on the progression of PD, especially in hypertensive animals, as evaluated by the resorption of the lower alveolar bone. This can be partly explained by the modulation in the expression of Angiotensin II receptors (AT1R and AT2R), reduced production of inflammatory mediators, the reduced expression of resorption markers, and the increased expression of the bone formation markers. (AU)

FAPESP's process: 17/02271-2 - The role of Telmisartan in osteoblastic differentiation in spontaneously hypertensive rats
Grantee:Mariana Sousa Patrocinio
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/23676-3 - MicroRNAs role in bone impairment induced by periodontal disease and the local renin-angiotensin system in spontaneous hypertensive rats
Grantee:Victor Gustavo Balera Brito
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/05873-3 - In vitro analysis of mast cells and renin-angiotensin system role of spontaneously hypertensive animals osteoblasts and osteoclasts
Grantee:Sabrina Cruz Tfaile Frasnelli
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/07095-8 - The role of mast cells upon the in vitro osteogenic differentiation of the mesenchymal stromal cells from the bone marrow of mice
Grantee:Maria Carolina Linjardi de Sousa
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
Grantee:Carlos Ferreira dos Santos
Support type: Research Projects - Thematic Grants