Autor(es)

Mussagy, Cassamo U. ^{[1, 2]} ; Santos-Ebinuma, Valeria C. ^[2] ; Kurnia, Kiki A. ^[3] ; Dias, V, Ana C. R. ; Carvalho, Pedro ^[1] ; Coutinho, Joao A. P. ^[1] ; Pereira, Jorge F. B. ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Aveiro, Aveiro Inst Mat, Dept Chem, CICECO, P-3810193 Aveiro - Portugal

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil

^[3] Univ Airlangga, Fac Fisheries & Marine, Dept Marine, Jalan Mulyorejo Kampus C, Surabaya 60115 - Indonesia

^[4] Univ Coimbra, CIEPQPF, Dept Chem Engn, Rua Silvio Lima, Polo 2, P-3030790 Coimbra - Portugal

• Tipo de documento: Artigo Científico
• Fonte: GREEN CHEMISTRY; v. 22, n. 23, p. 8478-8494, DEC 7 2020.
• Citações Web of Science: 0

Resumo

Natural bioactive compounds have been attracting growing interest from the industries as a ``greener{''} alternative to synthetic raw materials/products. Rhodotorula glutinis yeast naturally synthesizes added value compounds such as lipids and carotenoids, commonly used for cosmetic, pharmaceutical, and food applications. R. glutinis constitutes a rigid cell-wall structure, requiring energy-saving and efficient cell disruption methods for a sustainable recovery of the intracellular compounds. A simple and ecofriendly technology using mixed bio-based solvents (biosolvents) was evaluated here as an alternative platform to permeabilize yeast cells and to improve the selective recovery of beta-carotene, torularhodin, torulene and lipids. The extraction ability of pure and solvent mixtures (methanol, ethanol, ethyl acetate, isopropanol, cyclohexane and 2-methyl tetrahydrofuran) was initially screened, demonstrating the clear impact of using mixtures to improve the extraction yields (up to three-fold increase). After identifying ethyl acetate/ethanol/water as the solvent mixture with a greater capacity to extract carotenoids and lipids, the selective recovery of carotenoids and lipids was enhanced by optimizing the solvent mixture composition ratio. Envisioning the industrial application, an integrated biosolvent-based downstream platform was designed. Two different strategies were investigated to further isolate carotenoids and lipids from R. glutinis biomass and to recycle the ethyl acetate/ethanol/water mixture: (i) precipitation using cold acetone; (ii) sequential liquid-liquid extraction. The integrated process for each strategy was compared with a conventional extraction procedure in terms of recovery efficiencies and its environmental impact. Regardless of the strategy, it is shown that the mixture of ethyl acetate, ethanol and water (15/27/58% w/w) can be reused up to three consecutive extractive cycles, ensuring high extraction efficiency yields, while decreasing the process carbon footprint by about 75% compared to the conventional method. (AU)

• Processo FAPESP: 14/16424-7 - Otimização e aumento de escala do processo de extração líquido-líquido com líquidos iônicos (LIs) como ferramenta de separação sustentável do biofármaco antileucêmico L-Asparaginase (ASPase)
• Beneficiário: Jorge Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/50058-0 - Characterization and modelling of aqueous two-phase systems (atps) composed of ionic liquids (ils) and polymers:a boost towards develiping sustainable biopharmaceutical separion. (sprint 1/2015)
• Beneficiário: Jorge Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/19793-3 - Otimização e Aumento de Escala de Processos com Líquidos Iônicos como nova ferramenta de Purificação da Proteína Verde Fluorescente Recombinante produzida por Escherichia coli "GFPurIL"
• Beneficiário: Sandro Roberto Valentini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/50303-0 - Sustainable citrus waste biorefining as a source of value added bioproducts. (sprint 2/2017)
• Beneficiário: Valéria de Carvalho Santos Ebinuma
• Modalidade de apoio: Auxílio à Pesquisa - Regular