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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Integrative platform for the selective recovery of intracellular carotenoids and lipids from Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents

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Author(s):
Mussagy, Cassamo U. [1, 2] ; Santos-Ebinuma, Valeria C. [2] ; Kurnia, Kiki A. [3] ; Dias, V, Ana C. R. ; Carvalho, Pedro [1] ; Coutinho, Joao A. P. [1] ; Pereira, Jorge F. B. [2, 4]
Total Authors: 7
Affiliation:
[1] Univ Aveiro, Aveiro Inst Mat, Dept Chem, CICECO, P-3810193 Aveiro - Portugal
[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil
[3] Univ Airlangga, Fac Fisheries & Marine, Dept Marine, Jalan Mulyorejo Kampus C, Surabaya 60115 - Indonesia
[4] Univ Coimbra, CIEPQPF, Dept Chem Engn, Rua Silvio Lima, Polo 2, P-3030790 Coimbra - Portugal
Total Affiliations: 4
Document type: Journal article
Source: GREEN CHEMISTRY; v. 22, n. 23, p. 8478-8494, DEC 7 2020.
Web of Science Citations: 0
Abstract

Natural bioactive compounds have been attracting growing interest from the industries as a ``greener{''} alternative to synthetic raw materials/products. Rhodotorula glutinis yeast naturally synthesizes added value compounds such as lipids and carotenoids, commonly used for cosmetic, pharmaceutical, and food applications. R. glutinis constitutes a rigid cell-wall structure, requiring energy-saving and efficient cell disruption methods for a sustainable recovery of the intracellular compounds. A simple and ecofriendly technology using mixed bio-based solvents (biosolvents) was evaluated here as an alternative platform to permeabilize yeast cells and to improve the selective recovery of beta-carotene, torularhodin, torulene and lipids. The extraction ability of pure and solvent mixtures (methanol, ethanol, ethyl acetate, isopropanol, cyclohexane and 2-methyl tetrahydrofuran) was initially screened, demonstrating the clear impact of using mixtures to improve the extraction yields (up to three-fold increase). After identifying ethyl acetate/ethanol/water as the solvent mixture with a greater capacity to extract carotenoids and lipids, the selective recovery of carotenoids and lipids was enhanced by optimizing the solvent mixture composition ratio. Envisioning the industrial application, an integrated biosolvent-based downstream platform was designed. Two different strategies were investigated to further isolate carotenoids and lipids from R. glutinis biomass and to recycle the ethyl acetate/ethanol/water mixture: (i) precipitation using cold acetone; (ii) sequential liquid-liquid extraction. The integrated process for each strategy was compared with a conventional extraction procedure in terms of recovery efficiencies and its environmental impact. Regardless of the strategy, it is shown that the mixture of ethyl acetate, ethanol and water (15/27/58% w/w) can be reused up to three consecutive extractive cycles, ensuring high extraction efficiency yields, while decreasing the process carbon footprint by about 75% compared to the conventional method. (AU)

FAPESP's process: 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase)
Grantee:Jorge Pereira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/50058-0 - Characterization and modelling of aqueous two-phase systems (ATPS) composed of ionic liquids (ILs) and polymers: a boost towards develiping sustainable biopharmaceutical separion
Grantee:Jorge Pereira
Support Opportunities: Regular Research Grants
FAPESP's process: 14/19793-3 - Optimization and scale-up of novel Ionic-Liquid-based purification processes for recombinant green fluorescent protein produced by Escherichia coli "GFPurIL"
Grantee:Sandro Roberto Valentini
Support Opportunities: Regular Research Grants
FAPESP's process: 17/50303-0 - Sustainable citrus waste biorefining as a source of value added bioproducts
Grantee:Valéria de Carvalho Santos Ebinuma
Support Opportunities: Regular Research Grants