Biomphalaria glabrata infected with Angiostrongylus cantonensis: Proteomic changes in the snail host

Autor(es)

Mendes, Tiago M. F. ^[1] ; Carrilho, Emanuel ^[2] ; Galinaro, Carlos A. ^[2] ; Cabral, Fernanda J. ^[1] ; Allegretti, Silmara M. ^[1]

Afiliação do(s) autor(es)

^[1] Campinas State Univ UNICAMP, Anim Biol Dept, Biol Inst, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 212, DEC 2020.
• Citações Web of Science: 0

Resumo

Angiostrongylus cantonensis is the main cause of human eosinophilic meningitis. Humans are accidental hosts, becoming infected due to ingestion of raw intermediate (snails and slugs) or paratenic hosts. Once ingested, the larvae migrate towards the brain where they die, causing the disease. To develop better mollusk control strategies, it is important to first understand what happens in the snail during infection, therefore our purpose was to characterize proteomic, metabolic and immunologic changes in Biomphalaria glabrata 24 h after infection with A. cantonensis. For this purpose, proteins were extracted from infected and uninfected snails and analyzed through mass spectrometry. Hemolymph was also collected, the number of hemocytes was counted and urea, nitric oxide, calcium, glycogen levels as well as alanine and aspartate aminotransferases activities were assessed. The cephalopodal region and gonad-digestive gland coYmplex were dissected and their glycogen content was measured. After infection with A. cantonensis, we observed an increase of hemocytes and granulocytes as well as an increase in hemoglobin type 2 proteins. Temptin-like protein was also found up-regulated in infected snails. Several proteins with structural function (such as myosin heavy chain - striated muscle - like and protein LOC106059779 with ADAM/reprosolin domain) were also differentially expressed, suggesting loss/damage of internal tissues. Increase in phosphoglycerate mutase indicates an increase in glycolysis, possible to compensate the increase in energetic needs. Consequently, there is a decrease in glycogen reserves, particularly in the gonad - digestive gland complex. (AU)

• Processo FAPESP: 17/07364-9 - Estudo das modificações pós-traducionais de histonas e de co-reguladores da expressão nos estágios de vida do Schistosoma mansoni
• Beneficiário: Fernanda Janku Cabral
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07137-0 - Análise proteômica de Biomphalaria glabrata infectada por Schistosoma mansoni e Angiostrongylus cantonensis
• Beneficiário: Silmara Marques Allegretti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54040-8 - EMU: aquisição de um espectrômetro de massas de alta resolução Orbitrap para descoberta e elucidação estrutural compostos biologicamente ativos: aplicações em proteômica e biomarcadores, síntese, isolamento e caracterização de produtos naturais, estudos de sistemas redox em alimentos e síntese enzimática
• Beneficiário: Emanuel Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários