A novel ruthenium(II) gallic acid complex disrupts the actin cytoskeleton and inhibits migration, invasion and adhesion of triple negative breast tumor cells

Autor(es)

Graminha, Angelica E. ^{[1, 2]} ; Honorato, Joao ^[1] ; Correa, Rodrigo S. ^[3] ; Cominetti, Marcia R. ^[2] ; Menezes, Antonio C. S. ^[4] ; Batista, Alzir A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Dept Quim, Rodovia Washington Luis Km 235, CP 676, BR-13561001 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos UFSCar, Dept Gerontol, Rodovia Washington Luis Km 235, CP 676, BR-13561901 Sao Carlos, SP - Brazil

^[3] Univ Fed Ouro Preto UFOP, Dept Quim, BR-35400000 Ouro Preto, MG - Brazil

^[4] Univ Estadual Goias UEG, Ctr Ciencias Exatas & Tecnol, BR 153, CP 459, BR-75132903 Anapolis, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DALTON TRANSACTIONS; v. 50, n. 1, p. 323-335, JAN 7 2021.
• Citações Web of Science: 0

Resumo

This work describes the synthesis of three new ruthenium(II) complexes with gallic acid and derivatives of the general formula {[}Ru(L)(dppb)(bipy)]PF6, where L = gallate (GAC), benzoate (BAC), and esterified-gallate (EGA), bipy = 2,2 `-bipyridine and dppb = 1,4-bis(diphenylphosphino)butane. The complexes were characterized by elemental analysis, molar conductivity, NMR, cyclic voltammetry, UV-vis and IR spectroscopy, and two of them by X-ray crystallography. Cell viability assays show promising results, indicating higher cytotoxicity of the complexes in MDA-MB-231 cells, a triple-negative breast cancer (TNBC) cell line, compared with the hormone-dependent MCF-7 cell line. Studies in vitro with the MDA-MB-231 cell line showed that only Ru(BAC) and Ru(GAC) interacted with BSA. Besides that, the Ru(GAC) complex, which has a polyphenolic acid, interacted in an apo-Tf structure and function dependent manner and it was able to inhibit the formation of reactive oxygen species. Ru(GAC) was able to cause damage to the cellular cytoskeleton leading to inhibition of some cellular processes of TNBC cells, such as invasion, migration, and adhesion. (AU)

• Processo FAPESP: 14/19632-0 - Novos complexos de rutênio com ligantes bioativos: investigação do mecanismo de ação
• Beneficiário: Angelica Ellen Graminha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/16312-0 - Citotoxidade e mecanismo de ação de complexos de rutênio contendo produtos naturais ou derivados
• Beneficiário: Alzir Azevedo Batista
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24940-8 - Avaliação da eficácia de mudanças estruturais do [10]-gingerol em combinação com o quimioterápico doxorubicina para o tratamento de câncer de mama: estudos in vitro e in vivo
• Beneficiário: Márcia Regina Cominetti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs