Polarization, migration, and homotypical interactions among prostatic smooth muscle cells in a laminin 111-rich extracellular matrix

Autor(es)

Osorio, Daniel A. ^[1] ; Consonni, Silvio R. ^[2] ; dos Santos, Aline M. ^{[1, 3]} ; Carvalho, Hernandes F. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, UNICAMP, Carl von Linnaeus St S-N Infab Bldg, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, UNICAMP, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Natl Inst Photon Appl Cell Biol, UNICAMP, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 45, n. 4 JAN 2021.
• Citações Web of Science: 0

Resumo

Prostate cancer is a life-threatening condition worldwide. As the tumor progresses, smooth muscle cells (SMCs) become atrophic/dedifferentiated, within a series of stromal changes named stromal reaction. Here, we tested whether a laminin 111-rich extracellular matrix (Lr-ECM) could affect SMCs phenotype and differentiation status. Using time-lapse microscopy, image analyses, quantitative real-time reverse transcription polymerase chain reaction, immunohistochemistry and immunoblotting, and transmission electron microscopy, we showed that SMCs acquires a migratory behavior with a decreased expression of differentiation markers and relocation of focal adhesion kinase. SMCs set homotypic cell junctions and were active in autophagy/phagocytosis. Analysis of the migratory behavior showed that SMCs polarized and migrated toward each other, recognizing long-distance signals such as matrix tensioning. However, half of the cell population were immotile, irrespective of the nearest neighbor distance, suggesting they do not engage in productive interactions, possibly as a result of back-to-back positioning. In conclusion, the Lr-ECM, mimics the effects of the proliferating and infiltrating tumor epithelium, causing SMCs phenotypical change similar to that observed in the stromal reaction, in addition to a hitherto undescribed, stereotyped pattern of cell motility resulting from cell polarization. (AU)

• Processo FAPESP: 15/03235-4 - Células musculares lisas prostáticas: regulação da diferenciação e da ativação da quinase de adesão focal (FAK) pela interação com o epitélio e com a matriz extracelular
• Beneficiário: Daniel Andrés Osorio Rodríguez
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático