Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NF kappa B Activation and Cytokine Production

Autor(es):Mostrar menos -

Borghi, Sergio M. ^{[1, 2]} ; Fattori, Victor ^[1] ; Carvalho, Thacyana T. ^[1] ; Tatakihara, Vera L. H. ^[1] ; Zaninelli, Tiago H. ^[1] ; Pinho-Ribeiro, Felipe A. ^[1] ; Ferraz, Camila R. ^[1] ; Staurengo-Ferrari, Larissa ^[1] ; Casagrande, Rubia ^[3] ; Pavanelli, Wander R. ^[1] ; Cunha, Fernando Q. ^[4] ; Cunha, Thiago M. ^[4] ; Pinge-Filho, Phileno ^[1] ; Verri, Waldiceu A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Biol Sci, Dept Pathol, Londrina, Parana - Brazil

^[2] Univ Northern Parana Unopar, Ctr Res Hlth Sci, Londrina, Parana - Brazil

^[3] Univ Estadual Londrina, Univ Hosp, Hlth Sci Ctr, Dept Pharmaceut Sci, Londrina, Parana - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 11, JAN 26 2021.
• Citações Web of Science: 0

Resumo

The neglected tropical infirmity Chagas disease (CD) presents high mortality. Its etiological agent T. cruzi is transmitted by infected hematophagous insects. Symptoms of the acute phase of the infection include fever, fatigue, body aches, and headache, making diagnosis difficult as they are present in other illnesses as well. Thus, in endemic areas, individuals with undetermined pain may be considered for CD. Although pain is a characteristic symptom of CD, its cellular and molecular mechanisms are unknown except for demonstration of a role for peripheral TNF-alpha in CD pain. In this study, we evaluate the role of spinal cord glial cells in experimental T. cruzi infection in the context of pain using C57BL/6 mice. Pain, parasitemia, survival, and glial and neuronal function as well as NF kappa B activation and cytokine/chemokine production were assessed. T. cruzi infection induced chronic mechanical and thermal hyperalgesia. Systemic TNF-alpha and IL-1 beta peaked 14 days postinfection (p.i.). Infected mice presented increased spinal gliosis and NF kappa B activation compared to uninfected mice at 7 days p.i. Glial and NF kappa B inhibitors limited T. cruzi-induced pain. Nuclear phosphorylated NF kappa B was detected surrounded by glia markers, and glial inhibitors reduced its detection. T. cruzi-induced spinal cord production of cytokines/chemokines was also diminished by glial inhibitors. Dorsal root ganglia (DRG) neurons presented increased activity in infected mice, and the production of inflammatory mediators was counteracted by glial/NF kappa B inhibitors. The present study unveils the contribution of DRG and spinal cord cellular and molecular events leading to pain in T. cruzi infection, contributing to a better understanding of CD pathology. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs