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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Palmitic Acid Impairs Myogenesis and Alters Temporal Expression of miR-133a and miR-206 in C2C12 Myoblasts

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Autor(es):
da Paixao, Ailma O. [1] ; Bolin, Anaysa Paola [1] ; Silvestre, Joao G. [2] ; Rodrigues, Alice Cristina [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Pharmacol, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 5 MAR 2021.
Citações Web of Science: 0
Resumo

Palmitic acid (PA), a saturated fatty acid enriched in high-fat diet, has been implicated in the development of sarcopenic obesity. Herein, we chose two non-cytotoxic concentrations to better understand how excess PA could impact myotube formation or diameter without inducing cell death. Forty-eight hours of 100 mu M PA induced a reduction of myotube diameter and increased the number of type I fibers, which was associated with increased miR-206 expression. Next, C2C12 myotube growth in the presence of PA was evaluated. Compared to control cells, 150 mu M PA reduces myoblast proliferation and the expression of MyoD and miR-206 and miR-133a expression, leading to a reduced number and diameter of myotubes. PA (100 mu M), despite not affecting proliferation, impairs myotube formation by reducing the expression of Myf5 and miR-206 and decreasing protein synthesis. Interestingly, 100 and 150 mu M PA-treated myotubes had a higher number of type II fibers than control cells. In conclusion, PA affects negatively myotube diameter, fusion, and metabolism, which may be related to myomiRs. By providing new insights into the mechanisms by which PA affects negatively skeletal muscle, our data may help in the discovery of new targets to treat sarcopenic obesity. (AU)

Processo FAPESP: 15/24789-8 - MicroRNAs na via de sinalização de adiponectina: possíveis alvos terapêuticos na melhora da resistência à insulina e de doenças associadas.
Beneficiário:Alice Cristina Rodrigues
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/07087-8 - O papel dos microRNAs nas alterações metabólicas promovidas pela obesidade induzida por dieta ou por obesidade materna
Beneficiário:Alice Cristina Rodrigues
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 20/08049-2 - MicroRNAs circulantes como mediadores das adaptações metabólicas promovidas pelo exercício aeróbio na obesidade
Beneficiário:Alice Cristina Rodrigues
Modalidade de apoio: Auxílio à Pesquisa - Regular