Progesterone and vitamin D downregulate the activation of the NLRP1/ NLRP3 inflammasomes and TLR4-MyD88-NF-kappa B pathway in monocytes from pregnant women with preeclampsia

Autor(es)

Matias, Mariana Leticia ^[1] ; Romao-Veiga, Mariana ^[1] ; Ribeiro, Vanessa Rocha ^[1] ; Nunes, Priscila Rezeck ^[1] ; Gomes, Virginia Juliani ^[1] ; Devides, Amanda Carreira ^[1] ; Borges, Vera Therezinha ^[1] ; Romagnoli, Graziela Goretti ^[2] ; Peracoli, Jose Carlos ^[1] ; Peracoli, Maria Terezinha ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP - Brazil

^[2] Oeste Paulista Univ UNOESTE, Dept Hlth Sci, Jau, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 144, APR 2021.
• Citações Web of Science: 0

Resumo

This study evaluated the in vitro modulatory effect of progesterone (PG) and vitamin D (VD) on NLRP1/NLRP3 inflammasomes and TLR4/NF-kappa B pathway in monocytes from pregnant women with preeclampsia (PE). Monocytes from 20 preeclamptic and 20 normotensive (NT) pregnant women, and THP-1 cells were cultured with/without hyaluronan (HA), PG, or VD to determine gene and protein expression of TLR4 receptor, phosphorylated NF-kappa B, I kappa B alpha, TLR4, MYD88, NF-kappa B, NLRP1, NLRP3, caspase-1, IL-1 beta, IL-18, TNF-alpha, and IL-10. Higher endogenous activation of inflammatory genes and higher protein expression of TLR4 and NF-kappa B was detected in monocytes of PE group and decreased after PG or VD treatment. Monocyte from PE stimulated with HA increased while treatment with PG or VD decreased the expression of genes and proteins related to the inflammasomes. THP-1 cells showed a similar immune response profile as monocytes from PE. These results demonstrate that PG and VD play an immunomodulatory role in monocyte activation. (AU)

• Processo FAPESP: 15/26147-3 - Efeito modulador da progesterona, vitamina D e silibinina em monócitos de gestantes portadoras de pré-eclâmpsia
• Beneficiário: Mariana Leticia Matias
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/18155-9 - Modulação da resposta inflamatória sistêmica na pré-eclâmpsia
• Beneficiário: Maria Terezinha Serrão Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular