Step by Step about Germ Cells Development in Canine

Autor(es)

de Souza, Aline Fernanda ^[1] ; Pieri, Naira Caroline Godoy ^[1] ; Martins, Daniele dos Santos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: ANIMALS; v. 11, n. 3 MAR 2021.
• Citações Web of Science: 0

Resumo

Simple Summary The progression of germ cells is a remarkable event that allows biological discovery in the differ-entiation process during in vivo and in vitro development. This is crucial for understanding one toward making oogenesis and spermatogenesis. Companion animals, such as canine, could offer new animal models for experimental and clinical testing for translation to human models. In this review, we describe the latest and more relevant findings on germ cell development. In addition, we showed the methods available for obtaining germ cells in vitro and the characterization of pri-mordial germ cells and spermatogonial stem cells. However, it is necessary to further conduct basic research in canine to clarify the beginning of germ cell development. Primordial germ cells (PGCs) have been described as precursors of gametes and provide a connection within generations, passing on the genome to the next generation. Failures in the formation of gametes/germ cells can compromise the maintenance and conservation of species. Most of the studies with PGCs have been carried out in mice, but this species is not always the best study model when transposing this knowledge to humans. Domestic animals, such as canines (canine), have become a valuable translational research model for stem cells and therapy. Furthermore, the study of canine germ cells opens new avenues for veterinary reproduction. In this review, the objective is to provide a comprehensive overview of the current knowledge on canine germ cells. The aspects of canine development and germ cells have been discussed since the origin, specifications, and development of spermatogonial canine were first discussed. Additionally, we discussed and explored some in vitro aspects of canine reproduction with germ cells, such as embryonic germ cells and spermatogonial stem cells. (AU)

• Processo FAPESP: 12/05609-0 - Espermatogênese xenogênica pós-transplante de células tronco caninas no testículo de camundongos
• Beneficiário: Naira Caroline Godoy Pieri
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/15260-6 - Identificação e rota de migração das células germinativas primordiais caninas
• Beneficiário: Eloise Cristina de Ramos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/09148-7 - Caracterização de células multipotentes oriundas do folículo de penas como fonte para criação de um banco de recursos genéticos de aves silvestres
• Beneficiário: Daniele dos Santos Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/26761-0 - Reprogramação de células germinativas embrionárias humanas
• Beneficiário: Aline Fernanda de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/10542-5 - Epigenética da reprogramação em células germinativas embrionárias caninas
• Beneficiário: Aline Fernanda de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado