Salivary alpha-1-antitrypsin and macrophage migration inhibitory factor may be potential prognostic biomarkers for oncologic treatment-induced severe oral mucositis

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Palmier, Natalia Rangel ^[1] ; Paes Leme, Adriana Franco ^[2] ; De Rossi, Tatiane ^[2] ; Telles, Guilherme Pimentel ^[3] ; Morais-Faria, Karina ^[4] ; Kowalski, Luiz Paulo ^[5] ; Marta, Gustavo Nader ^{[6, 7]} ; Brandao, Thais Bianca ^{[4, 8]} ; Arany, Praveen R. ^[9] ; Migliorati, Cesar Augusto ^[10] ; Santos-Silva, Alan Roger ^[1] ; Prado-Ribeiro, Ana Carolina ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Oral Diag Dept, Piracicaba Dent Sch, Campinas - Brazil

^[2] CNPEM, Brazilian Biosci Natl Lab, LNBio, Campinas - Brazil

^[3] Univ Campinas UNICAMP, Inst Comp, Campinas - Brazil

^[4] ICESP FMUSP, Dent Oncol Serv, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Cent Inst, Hosp Clin, Med Sch FMUSP, Sao Paulo - Brazil

^[6] Hosp Sirio Libanes, Dept Radiat Oncol, Sao Paulo - Brazil

^[7] ICESP FMUSP, Dept Radiol & Oncol, Div Radiat Oncol, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil

^[8] Hosp Sirio Libanes, Oral Med Dept, Sao Paulo - Brazil

^[9] Univ Buffalo, Oral Biol & Biomed Engn, Buffalo, NY - USA

^[10] Univ Florida, Coll Dent, Gainesville, FL - USA

• Tipo de documento: Artigo Científico
• Fonte: SUPPORTIVE CARE IN CANCER; v. 29, n. 6, p. 2939-2946, JUN 2021.
• Citações Web of Science: 0

Resumo

Aims Evaluate the abundance of the selected targets, alpha-1-antitrypsin (A1AT) and macrophage migration inhibitory factor (MIF), and correlate these findings with the risk of developing severe oral mucositis (OM). Materials and methods Head and neck squamous cell carcinoma (HNSCC) patients submitted to radiotherapy (RT) or chemoradiotherapy (CRT) were assessed. OM grade and pain were evaluated daily during treatment. Two protein targets, A1AT and MIF, were evaluated, using selected reaction monitoring-mass spectrometry (SRM-MS), in whole saliva, collected prior to oncologic treatment. The results obtained from the targeted proteomic analysis were correlated with OM clinical outcomes. Results A total of 27 patients were included, of whom 21 (77.8%) had locally advanced disease (clinical stage III or IV). Most patients (70.4%) received CRT. OM grades 2 (40.8%) and 3 (33.3%) were the most prevalent during RT with a mean highest reported OM-related pain of 3.22 through the visual analogue scale (VAS). The abundance of A1AT and MIF correlated significantly with severe (grades 3 or 4,p < 0.02) compared with moderate-low (grades 1 or 2,p < 0.04) OM grade. Conclusions There is a correlation between the abundance of salivary A1AT and MIF and oncologic treatment-induced OM. The correlation of MIF expression with severe OM appears to be compatible with its physiological pro-inflammatory role. These results open up great possibilities for the use of salivary MIF and A1AT levels as prognostic markers for effective therapeutic interventions, such as photobiomodulation therapy, patient-controlled analgesia, or personalized medicaments. (AU)

• Processo FAPESP: 18/04657-8 - Proteínas salivares candidatas a marcadores de toxicidade bucal associada à radioterapia
• Beneficiário: Natália Rangel Palmier
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/12431-1 - Verificação de proteínas da saliva candidatas a marcadores em CEC e sua correlação com o prognóstico
• Beneficiário: Tatiane de Rossi Mazo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/06138-1 - Estudo prospectivo da incidência de trismo induzido por radioterapia em pacientes com carcinoma espinocelular de boca
• Beneficiário: Ana Carolina Prado Ribeiro e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18402-8 - Avaliação dos efeitos diretos da radiação sobre o esmalte, a dentina e a polpa dental de pacientes com câncer de cabeça e pescoço
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/22862-2 - Impacto da idade sobre as toxicidades bucais induzidas pela radioterapia em pacientes jovens com câncer de boca
• Beneficiário: Jaqueline de Lima Correia
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/07846-0 - Análise peptidômica de vesículas extracelulares originadas de linhagens celulares, saliva e plasma de pacientes com carcinoma de células escamosas da cavidade oral
• Beneficiário: Adriana Franco Paes Leme
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/02233-6 - Segurança oncológica no uso da fotobiomodulação intraoral e extraoral em pacientes com carcinoma espinocelular de cavidade oral e orofaringe
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular