Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway

Autor(es)

Santamarina, Aline B. ^[1] ; Pisani, Luciana P. ^[2] ; Baker, Ella J. ^[3] ; Marat, Andreea D. ^[3] ; Valenzuela, Carina A. ^{[3, 4]} ; Miles, Elizabeth A. ^[3] ; Calder, Philip C. ^{[5, 3, 6]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, BR-11015020 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biociencias, Lab Nutr & Fisiol Enddcrina LaNFE, BR-11015020 Santos, SP - Brazil

^[3] Univ Southampton, Fac Med, Sch Human Dev & Hlth, Southampton SO16 6YD, Hants - England

^[4] Univ Valparaiso, Fac Phannaty, Sch Nutr, Valparaiso - Chile

^[5] Univ Southampton, Southampton SO16 6YD, Hants - England

^[6] Univ Hosp Southampton NHS Fdn Trust, NIHR Southampton Biomed Res Ctr, Southampton SO16 6YD, Hants - England

• Tipo de documento: Artigo Científico
• Fonte: FOOD & FUNCTION; v. 12, n. 17 JUN 2021.
• Citações Web of Science: 0

Resumo

Monocyte recruitment and activation of macrophages are essential for homeostasis but are also related to the development and progression of cardiometabolic diseases. The management of inflammation with dietary components has been widely investigated. Two components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or the diet. Considering this, we aimed to analyze the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammation-related gene expression and protein concentrations of IL-1 beta, TNF-alpha, IL-6, MCP-1, and IL-10 were assessed. Also, NF kappa Bp65, I kappa B alpha, and PPAR-gamma protein expression were determined. OA, AC, and OAAC decreased pNF kappa Bp65, PPAR gamma, I kappa B alpha, TNF-alpha, IL-1 beta, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to control, OAAC decreased mRNA for TLR4, I kappa K alpha, I kappa B alpha, NF kappa B1, MCP-1, TNF-alpha, IL-6, and IL-1 beta more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with control, OA, and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect. (AU)

• Processo FAPESP: 18/23623-7 - Efeito da suplementação de ácido oléico e cianidinas em macrófagos na modulação de processos inflamatórios
• Beneficiário: Aline Boveto Santamarina
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/13875-0 - EFEITO DA SUPLEMENTAÇÃO DO FRUTO DA PALMEIRA JUÇARA (Euterpe edulis Mart.) NAS MODIFICAÇÕES EPIGENÉTICAS E ESTADO INFLAMATÓRIO EM MONÓCITOS DE ADULTOS OBESOS
• Beneficiário: Aline Boveto Santamarina
• Modalidade de apoio: Bolsas no Brasil - Doutorado