Bioaccessibility and Cellular Uptake of Carotenoids Extracted from Bactris gasipaes Fruit: Differences between Conventional and Ionic Liquid-Mediated Extraction

Autor(es)

de Souza Mesquita, Leonardo M. ^[1] ; Murador, Daniella Carisa ^[1] ; Neves, Bruna Vitoria ^[1] ; Cavalcante Braga, Anna Rafaela ^{[1, 2]} ; Pisani, Luciana Pellegrini ^[1] ; de Rosso, Veridiana Vera ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Silva Jardim St 136, BR-11015020 Santos, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Dept Exact & Earth Sci, Campus Diadema, BR-09972270 Diadema, SP - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Nutr & Food Serv Res Ctr, Silva Jardim St 136, BR-11015020 Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 26, n. 13 JUL 2021.
• Citações Web of Science: 0

Resumo

Currently, on an industrial scale, synthetic colorants are used in many fields, as well as those extracted with conventional organic solvents (COSs), leading to several environmental issues. Therefore, we developed a sustainable extraction and purification method mediated by ionic liquids (IL), which is considered an alternative high-performance replacement for COSs. Carotenoids are natural pigments with low bioaccessibility (BCT) and bioavailability (BV) but with huge importance to health. To investigate if the BCT and cellular uptake of the carotenoids are modified by the extraction method, we conducted a comparison assay between both extraction procedures (IL vs. COS). For this, we used the Amazonian fruit Bactris gasipaes, a rich source of pro-vitamin A carotenoids, to obtain the extract, which was emulsified and subjected to an in vitro digestion model followed by the Caco-2 cell absorption assay. The bioaccessibility of carotenoids using IL was better than those using COS (33.25%, and 26.84%, respectively). The cellular uptake of the carotenoids extracted with IL was 1.4-fold higher than those extracted using COS. Thus, IL may be a feasible alternative as extraction solvent in the food industry, replacing COS, since, in this study, no IL was present in the final extract. (AU)

• Processo FAPESP: 16/23242-8 - Extração de carotenóides de Bactris gasipaes com líquidos iônicos e sua aplicação em modelo de programação metabólica
• Beneficiário: Leonardo Mendes de Souza Mesquita
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/18910-1 - Líquidos Iônicos Como Extratores Verdes para Obtenção de Carotenoides: Estudo da Biodisponibilidade e Atividade Antioxidante
• Beneficiário: Veridiana Vera de Rosso
• Modalidade de apoio: Auxílio à Pesquisa - Regular