etection of Zika virus in urine from randomly tested individuals in Mirassol, Brazi

Autor(es)

Da Conceicao, Pamela Joyce Previdelli ^[1] ; De Carvalho, Lucas Rodrigues ^[1] ; De Godoy, Bianca Lara Venancio ^[2] ; Nogueira, Mauricio Lacerda ^[3] ; Terzian, Ana Carolina Bernardes ^[3] ; de Godoy, Moacir Fernandes ^[4] ; Calmon, Marilia Freitas ^[1] ; Bittar, Cintia ^{[1, 5]} ; Rahal, Paula ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Inst Biociencias Letras & Ciencias Exatas IBILCE, Lab Estudos Genom, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

^[3] Fac Med Sao Jose Do Rio Preto FAMERP, Lab Pesquisas Virol LPV, Sao Jose Do Rio Preto, SP - Brazil

^[4] Fac Med Sao Jose Do Rio Preto FAMERP, Dept Cardiol & Cardiovasc Surg, Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Estadual Paulista Unesp, Inst Biociencias Letras & Ciencias Exatas IBILCE, Lab Estudos Genom, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION; v. 50, n. 1 JUL 2021.
• Citações Web of Science: 1

Resumo

Purpose Studies show that around 80% of Zika virus (ZIKV) infections are asymptomatic. The present study tested urine samples from volunteers, unsuspected of arboviral infection, which attended an emergency care unit (ECU) in Mirassol, Brazil, from March 2018 to April 2019. Methods The volunteers were divided into two groups. The first group was composed of outpatients who were not suspected to have an arbovirus infection. This first group was subdivided into two subgroups: outpatients with and without arbovirus-like symptoms. The second group consisted of companions of outpatients treated at the ECU. The second group was also subdivided into two subgroups: totally asymptomatic individuals and those who had arbovirus-like symptoms. RNA was extracted from urine samples, followed by RT-qPCR for ZIKV. Results We found that 11% (79/697) of the samples tested positive for ZIKV-RNA. Among the ZIKV-RNA-positive individuals, 16.5% (13/79) were companions, of which 61.5% (8/13) were totally asymptomatic and 38.5% (5/13) reported symptoms that could be suggestive of arbovirus infection. In addition, 83.5% (66/79) of the ZIKV-RNA-positive individuals were outpatients without a clinical diagnosis of arbovirus. Of these undiagnosed ZIKV-RNA-positive outpatients, 47% (31/66) had no arbovirus-related symptoms. Conclusion Our study shows the effectiveness of urine as a non-invasive sample to detect the incidence of ZIKV infection. We also highlight the importance of ZIKV molecular diagnosis to aid public health surveillance and prevention of congenital Zika syndrome and other ZIKV-associated diseases. (AU)

• Processo FAPESP: 14/22198-0 - Potencial terapêutico de fármacos flavonóidicos na infecção com o vírus da hepatite C
• Beneficiário: Paula Rahal
• Modalidade de apoio: Auxílio à Pesquisa - Regular