Colorectal Cancer Cell-Derived Small Extracellular Vesicles Educate Human Fibroblasts to Stimulate Migratory Capacity

Autor(es)

Clerici, Stefano Piatto ^[1] ; Peppelenbosch, Maikel ^[2] ; Fuhler, Gwenny ^[2] ; Consonni, Silvio Roberto ^[1] ; Ferreira-Halder, Carmen Verissima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas - Brazil

^[2] Erasmus Univ, Dept Gastroenterol & Hepatol, Med Ctr Rotterdam, Rotterdam - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 9, JUL 15 2021.
• Citações Web of Science: 0

Resumo

Colorectal cancer (CRC) is in the top 10 cancers most prevalent worldwide, affecting equally men and women. Current research on tumor-derived extracellular vesicles (EVs) suggests that these small extracellular vesicles (sEVs) play an important role in mediating cell-to-cell communication and thus potentially affecting cancer progression via multiple pathways. In the present study, we hypothesized that sEVs derived from different CRC cell lines differ in their ability to reprogram normal human fibroblasts through a process called tumor education. The sEVs derived from CRC cell lines (HT29 and HCT116) were isolated by a combination of ultrafiltration and polymeric precipitation, followed by characterization based on morphology, size, and the presence or absence of EV and non-EV markers. It was observed that the HT29 cells displayed a higher concentration of sEVs compared with HCT116 cells. For the first time, we demonstrated that HT29-derived sEVs were positive for low-molecular-weight protein tyrosine phosphatase (Lmwptp). CRC cell-derived sEVs were uptake by human fibroblasts, stimulating migratory ability via Rho-Fak signaling in co-incubated human fibroblasts. Another important finding showed that HT29 cell-derived sEVs are much more efficient in activating human fibroblasts to cancer-associated fibroblasts (CAFs). Indeed, the sEVs produced by the HT29 cells that are less responsive to a cytotoxic agent display higher efficiency in educating normal human fibroblasts by providing them advantages such as activation and migratory ability. In other words, these sEVs have an influence on the CRC microenvironment, in part, due to fibroblasts reprogramming. (AU)

• Processo FAPESP: 15/20412-7 - Proteína tirosina fosfatase de baixo peso molecular em câncer de cólon retal: da bancada à geração de produto
• Beneficiário: Carmen Veríssima Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/03593-6 - Educação plaquetária e metástase: relevância da LMWPTP e vesículas extracelulares no Câncer Colorretal
• Beneficiário: Stefano Piatto Clerici
• Modalidade de apoio: Bolsas no Brasil - Doutorado