Lateral hypothalamus involvement in control of stress response by bed nucleus of the stria terminalis endocannabinoid neurotransmission in male rats

Autor(es)

Gomes-de-Souza, Lucas ^{[1, 2]} ; Costa-Ferreira, Willian ^{[1, 2]} ; Mendonca, Michelle M. ^[3] ; Xavier, Carlos H. ^[3] ; Crestani, Carlos C. ^{[1, 2, 4]}

Afiliação do(s) autor(es)

^[1] Joint UFSCar UNESP Grad Program Physiol Sci, Sao Carlos, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Lab Pharmacol, Araraquara, SP - Brazil

^[3] Univ Fed Goias, Inst Biol Sci, Goiania, Go - Brazil

^[4] S5o Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Dept Drugs & Pharmaceut, Lab Pharmacol, Rodovia Araraquara Jau Km 01, Campus Univ, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 AUG 9 2021.
• Citações Web of Science: 0

Resumo

The endocannabinoid neurotransmission acting via local CB1 receptor in the bed nucleus of the stria terminalis (BNST) has been implicated in behavioral and physiological responses to emotional stress. However, the neural network related to this control is poorly understood. In this sense, the lateral hypothalamus (LH) is involved in stress responses, and BNST GABAergic neurons densely innervate this hypothalamic nucleus. However, a role of BNST projections to the LH in physiological responses to stress is unknown. Therefore, using male rats, we investigated the role of LH GABAergic neurotransmission in the regulation of cardiovascular responses to stress by CB1 receptors within the BNST. We observed that microinjection of the selective CB1 receptor antagonist AM251 into the BNST decreased the number of Fos-immunoreactive cells within the LH of rats submitted to acute restraint stress. Treatment of the BNST with AM251 also enhanced restraint-evoked tachycardia. Nevertheless, arterial pressure increase and sympathetically-mediated cutaneous vasoconstriction to restraint was not affected by CB1 receptor antagonism within the BNST. The effect of AM251 in the BNST on restraint-evoked tachycardia was abolished in animals pretreated with the selective GABA(A) receptor antagonist SR95531 in the LH. These results indicate that regulation of cardiovascular responses to stress by CB1 receptors in the BNST is mediated by GABAergic neurotransmission in the LH. Present data also provide evidence of the BNST endocannabinoid neurotransmission as a mechanism involved in LH neuronal activation during stressful events. (AU)

• Processo FAPESP: 16/05028-9 - Controle das respostas cardiovasculares ao estresse pela neurotransmissão endocanabinóide no núcleo leito da estria terminal em ratos: interação com a via de sinalização receptor NMDA/NO/GMPc/proteína quinase g e envolvimento do hipotálamo lateral
• Beneficiário: Lucas Gomes de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/05218-2 - Envolvimento das sinalizações angiotensina II/receptor AT1 e angiotensina 1-7/ receptor Mas no núcleo medial da amígdala nas respostas cardiovasculares e ansiogênica ao estresse em ratos
• Beneficiário: Willian Costa Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/24478-3 - Estudo da participação do córtex insular no controle das respostas cardiovasculares e ansiogênica ao estresse em ratos: avaliação da topografia funcional e do envolvimento da neurotransmissão do fator de liberação de corticotrofina (CRF)
• Beneficiário: Carlos Cesar Crestani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/19249-0 - Envolvimento de neurotransmissões angiotensinérgicas do núcleo medial da amígdala no controle das respostas cardiovasculares e ansiogênica ao estresse em ratos.
• Beneficiário: Carlos Cesar Crestani
• Modalidade de apoio: Auxílio à Pesquisa - Regular