Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes

Texto completo
Autor(es):
Aparecida de Carvalho, Camila [1, 2] ; Mitsuyoshi Hiramoto, Roberto [3] ; Regina Meireles, Luciana [1] ; Franco de Andrade Junior, Heitor [1, 2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Protozool, Fac Med, Inst Med Trop, Av Doutor Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept Patol, Fac Med, Av Doutor Eneas Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil
[3] Adolfo Lutz Inst, Lab Parasitoses Sistem, Av Dr Arnaldo 355, BR-01246000 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Parasitology; v. 148, n. 12, p. 1509-1515, OCT 2021.
Citações Web of Science: 0
Resumo

Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two Leishmania (Leishmania) {[}L. (L.)] <full form> infantum antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan-bovine serum albumin (BSA) complex - GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research. (AU)

Processo FAPESP: 17/14675-0 - Resposta imune humoral a haptenos de L. (L.) infantum chagasi por detecção de anticorpos específicos e naturais na leishmaniose visceral experimental e natural
Beneficiário:Camila Aparecida de Carvalho
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/26558-9 - Haptenos na leishmaniose visceral. Desenvolvimento de ensaios de fase sólida para diagnóstico e sua imunologia em modelos experimentais.
Beneficiário:Heitor Franco de Andrade Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular