Inositol monophosphatase 1 (IMPA1) mutation in intellectual disability patients impairs neurogenesis but not gliogenesis

Autor(es):Mostrar menos -

Figueiredo, Thalita ^{[1, 2, 3]} ; Mendes, Ana P. D. ^[2] ; Moreira, Danielle P. ^[1] ; Goulart, Ernesto ^[1] ; Oliveira, Danyllo ^[1] ; Kobayashi, Gerson S. ^[1] ; Stern, Shani ^{[2, 4]} ; Kok, Fernando ^[1] ; Marchetto, Maria C. ^[2] ; Santos, Renata ^{[2, 5]} ; Gage, Fred H. ^[2] ; Zatz, Mayana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Human Genome & Stem Cell Res Ctr, Biosci Inst, 106 Rua Matao, BR-05508090 Sao Paulo - Brazil

^[2] Salk Inst Biol Studies, Lab Genet, 10010 North Torrey Pines Rd, La Jolla, CA 92037 - USA

^[3] Univ Fed Alagoas, Fac Med, BR-57072900 Maceio, Alagoas - Brazil

^[4] Univ Haifa, Fac Nat Sci, Sagol Dept Neurobiol, IL-3498838 Haifa - Israel

^[5] Univ Paris, IPNP, INSERM U1266, Lab Dynam Neuronal Struct Hlth & Dis, 102 Rue Sante, F-75014 Paris - France

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR PSYCHIATRY; v. 26, n. 7, p. 3558-3571, JUL 2021.
• Citações Web of Science: 0

Resumo

A homozygous mutation in the inositol monophosphatase 1 (IMPA1) gene was recently identified in nine individuals with severe intellectual disability (ID) and disruptive behavior. These individuals belong to the same family from Northeastern Brazil, which has 28 consanguineous marriages and 59 genotyped family members. IMPA1 is responsible for the generation of free inositol from de novo biosynthesis and recycling from inositol polyphosphates and participates in the phosphatidylinositol signaling pathway. To understand the role of IMPA1 deficiency in ID, we generated induced pluripotent stem cells (iPSCs) from patients and neurotypical controls and differentiated these into hippocampal dentate gyrus-like neurons and astrocytes. IMPA1-deficient neuronal progenitor cells (NPCs) revealed substantial deficits in proliferation and neurogenic potential. At low passage NPCs (P1 to P3), we observed cell cycle arrest, apoptosis, progressive change to a glial morphology and reduction in neuronal differentiation. These observations were validated by rescuing the phenotype with myo-inositol supplemented media during differentiation of patient-derived iPSCs into neurons and by the reduction of neurogenic potential in control NPCs-expressing shIMPA1. Transcriptome analysis showed that NPCs and neurons derived from ID patients have extensive deregulation of gene expression affecting pathways necessary for neurogenesis and upregulation of gliogenic genes. IMPA1 deficiency did not affect cell cycle progression or survival in iPSCs and glial progenitor cells or astrocyte differentiation. Therefore, this study shows that theIMPA1mutation specifically affects NPC survival and neuronal differentiation. (AU)

• Processo FAPESP: 16/09618-5 - Qual é o papel da enzima IMPA1 na Deficiência Intelectual Familial?
• Beneficiário: Thalita Cristina Figueiredo Cunha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/19877-0 - Compreender o papel da mutação no gene IMPA1 em neurônios hipocampais derivados de pacientes com deficiência intelectual familiar
• Beneficiário: Thalita Cristina Figueiredo Cunha
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 19/18469-1 - Desenvolvimento de organoides hepáticos universais produzidos a partir de células IPS
• Beneficiário: Ernesto da Silveira Goulart Guimarães
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50931-3 - INCT 2014 - Envelhecimento e Doenças Genéticas: Genômica e Metagenômica
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Temático