Spliced genes in muscle from Nelore Cattle and their association with carcass and meat quality

Autor(es):Mostrar menos -

Silva, Danielly B. S. ^[1] ; Fonseca, Larissa F. S. ^[1] ; Pinheiro, Daniel G. ^[1] ; Magalhaes, Ana F. B. ^[1] ; Muniz, Maria M. M. ^[1] ; Ferro, Jesus A. ^{[2, 1]} ; Baldi, Fernando ^{[2, 1]} ; Chardulo, Luis A. L. ^[3] ; Schnabel, Robert D. ^[4] ; Taylor, Jeremy F. ^[4] ; Albuquerque, Lucia G. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Jaboticabal, SP - Brazil

^[2] Natl Council Sci & Technol Dev CNPq, Brasilia, DF - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Vet & Anim Sci, Botucatu, SP - Brazil

^[4] Univ Missouri, Div Anim Sci, Columbia, MO - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 SEP 7 2020.
• Citações Web of Science: 6

Resumo

Transcript data obtained by RNA-Seq were used to identify differentially expressed alternatively spliced genes in ribeye muscle tissue between Nelore cattle that differed in their ribeye area (REA) or intramuscular fat content (IF). A total of 166 alternatively spliced transcripts from 125 genes were significantly differentially expressed in ribeye muscle between the highest and lowest REA groups (p <= 0.05). For animals selected on their IF content, 269 alternatively spliced transcripts from 219 genes were differentially expressed in ribeye muscle between the highest and lowest IF animals. Cassette exons and alternative 3 ` splice sites were the most frequently found alternatively spliced transcripts for REA and IF content. For both traits, some differentially expressed alternatively spliced transcripts belonged to myosin and myotilin gene families. The hub transcripts were identified for REA (LRRFIP1, RCAN1 and RHOBTB1) and IF (TRIP12, HSPE1 and MAP2K6) have an important role to play in muscle cell degradation, development and motility. In general, transcripts were found for both traits with biological process GO terms that were involved in pathways related to protein ubiquitination, muscle differentiation, lipids and hormonal systems. Our results reinforce the biological importance of these known processes but also reveal new insights into the complexity of the whole cell muscle mRNA of Nelore cattle. (AU)

• Processo FAPESP: 17/10630-2 - Aspectos genéticos da qualidade, eficiência e sustentabilidade da produção de carne em animais da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/16850-9 - Expressão diferencial de genes relacionados com caraterísticas da carcaça e carne de bovinos da raça Nelore
• Beneficiário: Danielly Beraldo dos Santos Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/22894-1 - Splicing alternativo de transcritos em tecidos musculares de bovinos da raça Nelore fenotipicamente divergente para características de carcaças e carne
• Beneficiário: Danielly Beraldo dos Santos Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 18/20026-8 - EMU concedido no processo 2017/10630-2: servidor
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários