Exercise Training Attenuates Ubiquitin-Proteasome Pathway and Increases the Genes Related to Autophagy on the Skeletal Muscle of Patients With Inflammatory Myopathies

Autor(es)

Pires Borges, Isabela Bruna ^[1] ; de Oliveira, Diego Sales ^[1] ; Nagahashi Marie, Suely Kazue ^[2] ; Lenario, Antonio Marcondes ^[3] ; Oba-Shinjo, Sueli Mieko ^[2] ; Shinjo, Samuel Katsuyuki ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Lab Inflammatory Myopathies, Div Rheumatol, Av Dr Arnaldo 455, Sala 3184, 3 Andar, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Neurol, Lab Mol & Cellular Biol, Sao Paulo - Brazil

^[3] Univ Michigan, Div Metab, Dept Internal Med Endocrinol & Diabet, Ann Arbor, MI - USA

• Tipo de documento: Artigo Científico
• Fonte: JCR-JOURNAL OF CLINICAL RHEUMATOLOGY; v. 27, n. 6, p. S224-S231, SEP 2021.
• Citações Web of Science: 0

Resumo

Background/Objective: The aim of this study was to evaluate the effects of exercise training on the ubiquitin-proteasome system (UPS) and genes related to autophagy on the skeletal muscle of patients with dermatomyositis (DM) and immune-mediated necrotizing myopathies (IMNMs). Methods: Seven DM patients and 6 IMNM patients were treated for 12 weeks with a twice-weekly aerobic and resistance training exercise program. Aerobic capacity, muscle strength, and expression of genes in the skeletal muscle related to UPS and to autophagy were evaluated at the baseline and after the intervention. Moreover, only at the baseline, 10 healthy control individuals were also evaluated. Results: The age of DM and IMNM patients was 49.8 and 58.5 years, respectively. Genes related to UPS were upregulated, whereas genes related to autophagy and antioxidative systems were downregulated only in the DM group when compared with control group. After completion of the exercise training program, several genes related to UPS were downregulated, whereas genes related to autophagy, mitochondrial pathways, and antioxidative systems were upregulated in both the DM and IMNM groups. Conclusions: Exercise training can increase genes related to autophagy, mitophagy, and lysosomal biogenesis in the skeletal muscle of patients. These results suggest an increase in the recycling of damaged proteins and organelles, which may also contribute to the performance and endurance of skeletal muscles in these patients. Furthermore, in patients with myositis, exercise training led to a decrease in genes related to UPS and an increase in genes related to antioxidative capacity. Therefore, this may also contribute to an attenuation of skeletal muscle loss and of the deleterious effects of oxidative stress on the skeletal muscle of these patients. (AU)

• Processo FAPESP: 16/19771-5 - Efeitos do treinamento físico no conteúdo lipídico e na resistência à ação da insulina do músculo esquelético de pacientes com miopatias inflamatórias idiopáticas: ensaio clínico controlado e randomizado
• Beneficiário: Diego Sales de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/11367-9 - Análise molecular e metabólica da Atorvastatina nos tecidos musculares de pacientes com Miopatias Autoimunes Sistêmicas
• Beneficiário: Isabela Bruna Pires Borges
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/13109-1 - Impacto do treinamento físico na resistência a ação da insulina, conteúdo lipídico, angiogênese e fibrogênese do músculo esquelético de pacientes com miopatias inflamatórias idiopáticas
• Beneficiário: Samuel Katsuyuki Shinjo
• Modalidade de apoio: Auxílio à Pesquisa - Regular