SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients

Autor(es):Mostrar menos -

da Silva, Lais Teodoro ^[1] ; Ortega, Marina Mazzilli ^[1] ; Tiyo, Bruna Tiaki ^[1] ; Tabosa Viana, Isabelle Freire ^[2] ; de Lima, Tayna Evily ^[2] ; Tozetto-Mendoza, Tania Regina ^[3] ; da Silva Oliveira, Luanda Mara ^[1] ; Emidio Teixeira, Franciane Mouradian ^[1] ; Lins, Roberto Dias ^[2] ; de Almeida, Alexandre ^[1] ; Mendes-Correa, Maria Cassia ^[3] ; da Silva Duarte, Alberto Jose ^{[1, 4]} ; Oshiro, Telma Miyuki ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med Dermatol & Imunodeficiencias LIM 5, Sao Paulo, SP - Brazil

^[2] Fundacao Oswaldo Cruz, Inst Aggeu Magalhaes, Dept Virol, Recife, PE - Brazil

^[3] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Virol LIM 52, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Div Lab Cent, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 76, 2021.
• Citações Web of Science: 0

Resumo

OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers. METHODS: Cellular immunity was assessed by intracellular detection of IFN-γ in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins. RESULTS: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-γ. CONCLUSIONS: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARS-CoV-2 proteins has been shown to be specific for the response of recovered individuals. (AU)

• Processo FAPESP: 18/12460-0 - Produção e caracterização de células dendríticas polarizantes aDC1 para protocolo clínico de imunoterapia anti-HIV
• Beneficiário: Laís Teodoro da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/25212-9 - Vacina terapêutica baseada em células dendríticas aDC1 e vírus autólogo inativado visando o controle viral de indivíduos infectados pelo HIV-1 em interrupção de terapia antirretroviral
• Beneficiário: Alberto José da Silva Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Temático