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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients

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Author(s):
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da Silva, Lais Teodoro [1] ; Ortega, Marina Mazzilli [1] ; Tiyo, Bruna Tiaki [1] ; Tabosa Viana, Isabelle Freire [2] ; de Lima, Tayna Evily [2] ; Tozetto-Mendoza, Tania Regina [3] ; da Silva Oliveira, Luanda Mara [1] ; Emidio Teixeira, Franciane Mouradian [1] ; Lins, Roberto Dias [2] ; de Almeida, Alexandre [1] ; Mendes-Correa, Maria Cassia [3] ; da Silva Duarte, Alberto Jose [1, 4] ; Oshiro, Telma Miyuki [1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med Dermatol & Imunodeficiencias LIM 5, Sao Paulo, SP - Brazil
[2] Fundacao Oswaldo Cruz, Inst Aggeu Magalhaes, Dept Virol, Recife, PE - Brazil
[3] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Virol LIM 52, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Div Lab Cent, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Clinics; v. 76, 2021.
Web of Science Citations: 0
Abstract

OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers. METHODS: Cellular immunity was assessed by intracellular detection of IFN-γ in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins. RESULTS: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-γ. CONCLUSIONS: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARS-CoV-2 proteins has been shown to be specific for the response of recovered individuals. (AU)

FAPESP's process: 18/12460-0 - Production and characterization of polarized dendritic cells aDC1 for use in anti-HIV immunotherapy
Grantee:Laís Teodoro da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/25212-9 - Therapeutic vaccine based on aDC1 dendritic cells pulsed with inactivated autologous virus for the control of viremia after ATI in HIV infected individuals
Grantee:Alberto José da Silva Duarte
Support Opportunities: Research Projects - Thematic Grants