Distinct Effects of the Hippocampal Transplantation of Neural and Mesenchymal Stem Cells in a Transgenic Model of Alzheimer's Disease

Autor(es)

Campos, Henrique C. ^[1] ; Ribeiro, Deidiane Elisa ^[2] ; Hashiguchi, Debora ^{[1, 3]} ; Hukuda, Deborah Y. ^[1] ; Gimenes, Christiane ^[1] ; Romariz, Simone A. A. ^[1] ; Ye, Qing ^{[2, 4, 5]} ; Tang, Yong ^{[4, 5]} ; Ulrich, Henning ^{[2, 4]} ; Longo, Beatriz Monteiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Fisiol, Lab Neurofisiol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil

^[3] Univ Fed Rio Grande do Norte, Brain Inst, Lab Plasticidade Sinapt, Caixa Postal 1524, BR-59078900 Natal, RN - Brazil

^[4] Chengdu Univ Tradit Chinese Med, Int Collaborat Ctr Big Sci Plan Purinerg Signalli, Chengdu 610075 - Peoples R China

^[5] Acupuncture & Chronobiol Key Lab Sichuan Prov, Chengdu 610075 - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: STEM CELL REVIEWS AND REPORTS; JAN 2022.
• Citações Web of Science: 0

Resumo

Alzheimer's disease (AD) is a severe disabling condition with no cure currently available, which accounts for 60-70% of all dementia cases worldwide. Therefore, the investigation of possible therapeutic strategies for AD is necessary. To this end, animal models corresponding to the main aspects of AD in humans have been widely used. Similar to AD patients, the double transgenic APPswe/PS1dE9 (APP/PS1) mice show cognitive deficits, hyperlocomotion, amyloid-beta (A beta) plaques in the cortex and hippocampus, and exacerbated inflammatory responses. Recent studies have shown that these neuropathological features could be reversed by stem cell transplantation. However, the effects induced by neural (NSC) and mesenchymal (MSC) stem cells has never been compared in an AD animal model. Therefore, the present study aimed to investigate whether transplantation of NSC or MSC into the hippocampus of APP/PS1 mice reverses AD-induced pathological alterations, evaluated by the locomotor activity (open field test), short- and long-term memory (object recognition) tests, A beta plaques (6-E10), microglia distribution (Iba-1), M1 (iNOS) and M2 (ARG-1) microglial phenotype frequencies. NSC and MSC engraftment reduced the number of A beta plaques and produced an increase in M2 microglia polarization in the hippocampus of APP/PS1 mice, suggesting an anti-inflammatory effect of stem cell transplantation. NSC also reversed the hyperlocomotor activity and increased the number of microglia in the hippocampus of APP/PS1 mice. No impairment of short or long-term memory was observed in APP/PS1 mice. Overall, this study highlights the potential beneficial effects of transplanting NSC or MSC for AD treatment. (AU)

• Processo FAPESP: 17/12412-2 - Processos degenerativos e regenerativos na Doença de Alzheimer: da cultura de células à terapia celular
• Beneficiário: Beatriz de Oliveira Monteiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/07366-4 - Receptores de purinas e cininas como alvos de estudo e intervenção terapêutica em doenças neurológicas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático