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A Genetic Model of Epilepsy with a Partial Alzheimer's Disease-Like Phenotype and Central Insulin Resistance

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Alves, Suelen Santos ; Patricio da Silva Junior, Rui Milton ; Delfino-Pereira, Polianna ; Alves Goulart Pereira, Marilia Gabriella ; Vasconcelos, Israel ; Schwaemmle, Hanna ; Mazzei, Rodrigo Focosi ; Carlos, Maiko Luiz ; Espreafico, Enilza Maria ; Tedesco, Antonio Claudio ; Sebollela, Adriano ; Almeida, Sebastiao Sousa ; Cortes de Oliveira, Jose Antonio ; Garcia-Cairasco, Norberto
Número total de Autores: 14
Tipo de documento: Artigo Científico
Fonte: Molecular Neurobiology; v. 59, n. 6, p. 17-pg., 2022-04-04.
Resumo

Studies have suggested an important connection between epilepsy and Alzheimer's disease (AD), mostly due to the high number of patients diagnosed with AD who develop epileptic seizures later on. However, this link is not well understood. Previous studies from our group have identified memory impairment and metabolic abnormalities in the Wistar audiogenic rat (WAR) strain, a genetic model of epilepsy. Our goal was to investigate AD behavioral and molecular alterations, including brain insulin resistance, in naive (seizure-free) animals of the WAR strain. We used the Morris water maze (MWM) test to evaluate spatial learning and memory performance and hippocampal tissue to verify possible molecular and immunohistochemical alterations. WARs presented worse performance in the MWM test (p < 0.0001), higher levels of hyperphosphorylated tau (S396) (p < 0.0001) and phosphorylated glycogen synthase kinase 3 (S21/9) (p < 0.05), and lower insulin receptor levels (p < 0.05). Conversely, WARs and Wistar controls present progressive increase in amyloid fibrils (p < 0.0001) and low levels of soluble amyloid-p. Interestingly, the detected alterations were age-dependent, reaching larger differences in aged than in young adult animals. In summary, the present study provides evidence of a partial AD-like phenotype, including altered regulation of insulin signaling, in a genetic model of epilepsy. Together, these data contribute to the understanding of the connection between epilepsy and AD as comorbidities. Moreover, since both tau hyperphosphorylation and altered insulin signaling have already been reported in epilepsy and AD, these two events should be considered as important components in the interconnection between epilepsy and AD pathogenesis and, therefore, potential therapeutic targets in this field. (AU)

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Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50891-1 - INCT 2014: Translacional em Medicina
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Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
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Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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