| Texto completo | |
| Autor(es): Mostrar menos - |
Pontelli, Marjorie C.
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Castro, Italo A.
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Martins, Ronaldo B.
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La Serra, Leonardo
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Veras, Flavio P.
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Nascimento, Daniele C.
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Silva, Camila M.
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Cardoso, Ricardo S.
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Rosales, Roberta
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Gomes, Rogerio
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Lima, Thais M.
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Souza, Juliano P.
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Vitti, Brenda C.
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Caetite, Diego B.
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de Lima, Mikhael H. F.
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Stumpf, Spencer D.
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Thompson, Cassandra E.
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Bloyet, Louis-Marie
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Toller-Kawahisa, Juliana E.
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Giannini, Marcela C.
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Bonjorno, Leticia P.
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Lopes, Maria I. F.
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Batah, Sabrina S.
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Li Siyuan
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Luppino-Assad, Rodrigo
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Almeida, Sergio C. L.
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Oliveira, Fabiola R.
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Benatti, Maira N.
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Pontes, Lorena L. F.
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Santana, Rodrigo C.
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Vilar, Fernando C.
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Auxiliadora-Martins, Maria
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Shi, Pei-Yong
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Cunha, Thiago M.
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Calado, Rodrigo T.
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Alves-Filho, Jose C.
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Zamboni, Dario S.
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Fabro, Alexandre T.
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Louzada-Junior, Paulo
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Oliveira, Rene D. R.
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Whelan, Sean P. J.
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Cunha, Fernando Q.
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Arruda, Eurico
Número total de Autores: 43
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| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF MOLECULAR CELL BIOLOGY; v. 14, n. 4, p. 14-pg., 2022-08-17. |
| Resumo | |
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, a hallmark that appears as both signature and prognosis of disease severity outcome. Although cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion, it is still unclear whether direct SARS-CoV-2 infection of immune cells could also play a role in this scenario by harboring viral replication. We found that monocytes, as well as both B and T lymphocytes, were susceptible to SARS-CoV-2 infection in vitro, accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell apoptosis. In addition, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019 (COVID-19) patients. The rates of SARS-CoV-2-infected monocytes in peripheral blood mononuclear cells from COVID-19 patients increased over time from symptom onset, with SARS-CoV-2-positive monocytes, B cells, and CD4(+) T lymphocytes also detected in postmortem lung tissue. These results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in COVID-19 patients might have important implications for disease pathogenesis and progression, immune dysfunction, and virus spread within the host. (AU) | |
| Processo FAPESP: | 14/02438-6 - Estudos com Bunyaviridae causadores de doença |
| Beneficiário: | Luiz Tadeu Moraes Figueiredo |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/16349-2 - Estudos sobre infecções persistentes por Paramyxovirus respiratórios humanos |
| Beneficiário: | Eurico de Arruda Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |